文献类型：期刊文献

英文题名：Production and optimization of recombinant human augmenter of liver regeneration by Pichia pastoris

作者：Li, Zhimin[1]; Ping, Xubin[1]; Ye, Qin[1]; Huang, Xiudong[2]; Cao, Zhifang[2]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Shanghai Wanxing Bio-Pharmaceutical Co. Ltd, Shanghai 201206, China

年份：2010

卷号：47

期号：5

起止页码：222

外文期刊名：Enzyme and Microbial Technology

收录：EI(收录号：20103513193071)

语种：英文

外文关键词：Fermentation - Yeast - Feeding

摘要：In this study, the fermentation of recombinant Pichia pastoris, which expresses heterogeneous human augmenter of liver regeneration (rhALR), has been optimized in a 5-L fermentor. The optimal methanol feeding method to optimize rhALR production was identified. Three methods of methanol feeding, methanol-stat, DO-stat and exponential feeding, were investigated to identify the method that would optimize rhALR production. It was determined that the methanol-stat method resulted in increased rhALR production, compared to the other two methods, which limit the amount of methanol available. Additionally, the results showed that rhALR expression levels could be enhanced by maintaining the pH at 6 and lowering the temperature from 30 to 25°C. Supplementation with riboflavin during the induction phase also increased rhALR production. After these optimizations, rhALR was produced at a concentration of 424. mg/L, a three-fold improvement over original process. ? 2010 Elsevier Inc.