文献类型：期刊文献

英文题名：Fluorescent "AND " logic gates for simultaneous detection of thiols and protons: photophysical properties, mechanism and bioimaging of living cells

作者：Zhou, Langping[1];Yu, Zhihao[1];Zhang, Guangzhu[1];Jin, Zechen[1,2];Zhang, Weibing[1];Qian, Junhong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Materia Med, Shanghai, Peoples R China

年份：2023

卷号：15

期号：6

起止页码：818

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20230613551834);WOS:【SCI-EXPANDED(收录号:WOS:000920202600001)】；

基金：This work was financially supported by NSFC (21974045).

语种：英文

外文关键词：Bioimaging - Charge transfer - Computer circuits - Logic gates - Probes

摘要：Five fluorescent probes TP1-5 were demonstrated as two-input "AND " molecular logic gates for the detection of thiols and protons. The molecules were designed based on "thiol receptor-spacer(1)-fluorophore-spacer(2)-proton receptor " mode. The logic gates were constructed by employing maleimide, naphthalimide and morpholine (TP1-3)/N-methyl piperazine (TP4-5) as the thiol receptor, fluorophore and proton receptor, respectively. All probes show significant fluorescence enhancements upon addition of both protons and thiols. However, much weaker spectral responses were observed with the addition of only one single analyte. The fluorescence outputs, based on photoinduced electron transfer (PET) and (twisted) intramolecular charge transfer (TICT/ICT), were modulated by the proton receptor and linker. The length of spacer(1) affects the responses toward thiols, whereas spacer(2) influences the sensing performance toward protons. The difference between the pK(a) values of morpholine (similar to 5.80) and N-methyl piperazine (similar to 7.10) enables us to detect thiols in divergent pH circumstances. TP1-3 exhibit an excellent "AND " logic function for simultaneous detection of protons and thiols as well as bioimaging thiols in weakly acidic living cells. However, TP4 and TP5 are not good candidates for executing "AND " logic operation possibly due to the stronger electron donating properties and steric effect of N-methyl piperazine.