文献类型：期刊文献

英文题名：Cervine genotype is the major Cryptosporidium genotype in sheep in China

作者：Wang, Yongli[1];Feng, Yaoyu[2];Cui, Bin[1];Jian, Fuchun[1];Ning, Changshen[1];Wang, Rongjun[1];Zhang, Longxian[1];Xiao, Lihua[3]

机构：[1]Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China;[2]E China Univ Sci & Technol, Sch Resource & Environm Engn, Shanghai 200237, Peoples R China;[3]Ctr Dis Control & Prevent, Div Parasit Dis, Natl Ctr Zoonot Vector Borne & Enter Dis, US Dept Hlth & Human Serv, Atlanta, GA 30341 USA

年份：2010

卷号：106

期号：2

起止页码：341

外文期刊名：PARASITOLOGY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000273035000005)】；

基金：This study was supported in part by the National Natural Science Foundation of China (number 30771881, 30871863, and 30928019), Henan Province Great Special Fund of Public Welfare (Henan Province Financial Administration number 2008-145), and Ministry of Health Special Funds of Public Sector Research (number 200808012). We thank Professors Tengyun Gao, Ming Li, and Feng Lin for their assistance in sample collection. We also thank Heping Dong, Qingbin Lu, Gengqu Gao, Chaofeng Ma, and Ke Shi for technical assistance.

语种：英文

摘要：To identify Cryptosporidium species/genotypes in sheep in China and to elucidate the endemic transmission of cryptosporidiosis, a total of 1,701 fecal samples from five farms in four prefectures in Henan Province (central China) were examined. Eighty-two Cryptosporidium-positive samples were analyzed by polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis of the small subunit (SSU) rRNA gene and PCR analysis of the 60 kDa glycoprotein (gp60) gene, and 41 were further analyzed by DNA sequencing of the PCR products. The SSU rRNA-based PCR identified two Cryptosporidium species and one genotype, including the Cryptosporidium cervine genotype (74/82), Cryptosporidium andersoni (4/82), and Cryptosporidium xiaoi (4/82). The cervine genotype was found in all age groups, C. xiaoi in lambs, and C. andersoni in ewes. There were intragenetic differences in the SSU rRNA gene sequences of the Cryptosporidium cervine genotype and C. xiaoi. No Cryptosporidium parvum was detected by both SSU rRNA- and gp60-based PCR assays. These findings suggest that sheep are a potential source for zoonotic infections of the Cryptosporidium cervine genotype.