文献类型：期刊文献

英文题名：Porous composite materials driven by carbohydrate-carbohydrate interactions for efficient enrichment of intact glycopeptides in liver cancer cells

作者：Song, Zihan[1];Ti, Wengeng[1];Liu, Meiyan[1];Liu, Fuxing[1];Zhang, Xiaoyu[3];Qing, Guangyan[3];Sheng, Qianying[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China

年份：2026

卷号：301

外文期刊名：TALANTA

收录：;EI(收录号：20255219803809);WOS:【SCI-EXPANDED(收录号:WOS:001653260400003)】；

基金：This work was supported by the Natural Science Foundation of Shanghai (25ZR1402105), the Fundamental Research Funds for the Central Universities, China (JKJ01251717).

语种：英文

外文关键词：N-glycopeptides; Liver cancer; SMMC-7721 cells; Nanomaterials; Carbohydrate-carbohydrate interactions

摘要：Aberrant N-glycosylation plays a key role in liver cancer progression, making glycopeptides promising biomarker candidates. With the increasing awareness of the potential for novel types and features of glycopeptides, there is a pressing need for advanced enrichment materials to facilitate their sensitive and specific detection. Here, we report the development of a hydrophilic material (DMSN-CS-LA) based on carbohydrate-carbohydrate interactions (CCI), which involves coating chitosan-lactobionic acid (CS-LA) onto the surface of dendritic mesoporous silica. DMSN-CS-LA exhibited outstanding performance in glycopeptide enrichment, with a low detection limit (2 fmol/mu L), remarkable identification selectivity (IgG:BSA = 1:500), and an ideal loading capacity (300 mg/g). Significantly, this study reports the first application of a solid-phase enrichment material to hepatocellular carcinoma using the SMMC-7721 cell line. The results showed that 455 N-glycopeptides corresponding to 187 glycoproteins were enriched from 150 mu g SMMC-7721 cell digests. Gene Ontology (GO) analysis linked the enriched N-glycopeptides to liver cancer development. Simultaneous capture of biomarkers including Lamin-B1, Transferrin receptor protein 1, Fatty acid-binding protein 5, folate receptor 1, and Aminopeptidase N, underscores the method's potential for early diagnosis and prognosis. Overall, DMSN-CS-LA offers an effective enrichment platform, advancing glycoproteomic analysis in cancer research.