文献类型：期刊文献

英文题名：Optimization of SCF feeding regimen for ex vivo expansion of cord blood hematopoietic stem cells

作者：Du, Zheng[1];Cai, Haibo[1];Ye, Zhaoyang[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：164

期号：2

起止页码：211

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20131316150158);WOS:【SCI-EXPANDED(收录号:WOS:000317995800006)】；

基金：This work was financially supported by the National Nature Science Foundation of China (20776043), the Key Project of Medicine of Shanghai (074319109) and the National High Technology Research and Development Program of China (863 Program) (2011AA020119).

语种：英文

外文关键词：Hematopoietic stem cells; Cord blood; Stem cell factor; Ex vivo expansion; Feeding regimen

摘要：Stem cell factor (SCF) plays important roles in ex vivo expansion of hematopoietic stem cells (HSCs). In this study, the effects of dose and feeding time of SCF on ex vivo expansion of CD34(+) cells were investigated in serum-free medium supplemented with a cytokine cocktail composed of SCF, thrombopoietin (TPO) and flt3-ligand (FL). Among the four tested doses (0, 5, 50 and 500 ng/mL), a SCF dose of 50 ng/mL was demonstrated to be most favorable for ex vivo expansion of CD34(+) cells, which resulted in 34.22 +/- 10.80 and 8.89 +/- 1.25 folds of expansion regarding total cells and CD34(+) cells, respectively. Meanwhile, the specific growth rate of cells, the consumption rate of SCF and the percentage of CD34(+)c-kit(+) cells during the 21-day culture process were analyzed. The results indicated that initial 4-day period was a critical stage for SCF functioning on CD34(+) cells during ex vivo expansion. Based on this, a modified SCF feeding regimen was proposed, in which SCF (50 ng/mL) was only supplemented on day 0 in the cytokine cocktail and cells were then fed with TPO and FL till the end of culture. It was found that this SCF feeding regimen could expand CD34(+) cells efficiently, thus providing a cost-effect expansion protocol for HSCs. (C) 2012 Elsevier B.V. All rights reserved.