文献类型：期刊文献

英文题名：Transcriptomic response of Ralstonia solanacearum to antimicrobial Pseudomonas fluorescens SN15-2 metabolites

作者：Lou, Haibo[1];Wang, Xiaobing[1];Chen, Jun[1];Wang, Bozhi[2];Wang, Wei[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Fudan Univ, Sch Informat Sci & Technol, Shanghai 200433, Peoples R China

年份：2018

卷号：64

期号：11

起止页码：816

外文期刊名：CANADIAN JOURNAL OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000448727200005)】；

基金：We thank Tao Li for kindly providing R. solanacearum. This study was supported by the National Key Research and Development Program of China (grants 2017YFD0200400, 2017YFD0201100) and the Project of Prospering Agriculture through Science and Technology of Shanghai, China (grant Hu NongKeTuiZi (2017) No. 2-2).

语种：英文

外文关键词：Pseudomonas fluorescens metabolites; Ralstonia solanacearum; R. solanacearum morphology; transcriptome

摘要：To develop efficient biocontrol agents, it is essential to investigate the response of soil-borne plant pathogens to such agents. For example, the response of Ralstonia solanacearum, the tomato wilt pathogen, to antimicrobial metabolites of Pseudomonas fluorescens is unknown. Thus, we assessed the effects of P. fluorescens SN15-2 fermentation broth on R. solanacearum by transmission electron microscopy and transcriptome technology. RNA sequencing identified 109 and 155 genes that are significantly upregulated and downregulated, respectively, in response to P. fluorescens metabolites, many of which are associated with the cell membrane and cell wall, and with nucleotide acid metabolism, iron absorption, and response to oxidative stress. This study highlights the effectiveness of P. fluorescens metabolites against the tomato wilt pathogen and helps clarify the underlying molecular mechanisms.