文献类型：期刊文献

英文题名：Lipid extraction from oleaginous yeast cells with very high lipid content, low cell density, and fragile cell wall

作者：Wang, Ya[1,2];Zhang, Bingyan[1];Principe, Umutumwa E.[1];Zhang, Bin[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shihezi Univ, Sch Chem & Chem Engn, Beisi Rd, Shihezi 832000, Xinjiang, Peoples R China

年份：2026

卷号：142

期号：1

起止页码：77

外文期刊名：JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录：;EI(收录号：20261320394810);WOS:【SCI-EXPANDED(收录号:WOS:001768535600002)】；

基金：This research was supported by the National Key R&D Program of China (2023YFA0914400) , the National Natural Science Foun-dation of China (32301269) , and the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900) . The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

语种：英文

外文关键词：Heat-assisted ultrasonic extraction; Modified Folch method; Oleaginous yeast; Microbial lipid]

摘要：The newly developed oleaginous yeast cells behave high lipid content, low cell density, and fragile cell walls, as well as partial cell disruption. To extract the microbial lipid from these very high lipid containing cells, the regular Folch method does not work because the cells are unable to be completely harvested by centrifugation due to the low cell density (lower than broth), and lipid leakage into the broth from broken cells. This study developed a heat-assisted ultrasonic method for direct lipid extraction from the liquid fermentation broth after incubated at 80 degrees C followed by ultrasonic treatment. The method significantly improved the lipid extraction yield by 12 %-42 % of the very high lipid containing Trichosporon cutaneum cells (45 %-75 %). The method also preserved unsaturated fatty acids more effectively than the modified Folch method. The calculation on cell dry weight and lipid content in cells verified the effectiveness of the method with acceptable derivations. This study offers a simple and reliable method for quantitative measurement of microbial lipids from oleaginous yeast cells with very high lipid content. (c) 2026, The Society for Biotechnology, Japan. All rights are reserved, including those for text and data mining, AI training, and similar technologies.