文献类型：期刊文献

中文题名：反相高效液相色谱法测定丙硫菌唑的含量

英文题名：Determination of Prothioconazole by RP-HPLC

作者：徐仲玉[1];周翾[1];马红梅[1];曾仲武[2];王美娟[1];廖道华[1]

机构：[1]华东理工大学药学院,上海200237;[2]浙江禾本农药化学有限公司,浙江温州325008

年份：2009

卷号：48

期号：9

起止页码：656

中文期刊名：农药

外文期刊名：Agrochemicals

收录：CSTPCD;;北大核心:【北大核心2008】；

语种：中文

中文关键词：反相高效液相色谱;丙硫菌唑;杀菌剂

外文关键词：rP-hPlc; prothioconazole; fungicide;

摘要：建立了反相高效液相色谱测定丙硫菌唑的方法。采用反相C18烷基柱,以乙腈-0.01mol/L碳酸铵缓冲盐(体积比60:40)为流动相,柱温为25℃,流速为1.0mL/min,采用VWD紫外检测器在230nm处进行检测。丙硫菌唑质量浓度为0.04～0.40g/L时,其峰面积与质量浓度的线性关系良好,线性相关系数为1.0000;平均回收率高于99.3%,相对标准偏差低于0.9%。
A method was developed for the quantitative determination of prothioconazole using HPLC. The sample was separated on a Diamocil c18 column at the temperature of 25 °c with the elution of acetonitrile and 0.01 mol/l salvolatile buffer （60：40, by vol） as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was 230 nm. The assay exhibited a good linearity in prothioconazole concentration range from 0.04 to 0.40 g/L with a correlation coefficient of 1.0000. the recovery was more than 99.3%, and the relative standard deviation was lower than 0.9%.