文献类型：期刊文献

英文题名：Complete genome sequence and genome-wide transposon mutagenesis enable the determination of genes required for sodium hypochlorite tolerance and drug resistance in pathogen Aeromonas veronii GD2019

作者：Bu, Yifan[1];Liu, Chengyu[1];Liu, Yabo[1];Yu, Wensong[4];Lv, Tingjin[4];Zhang, Yuanxing[2,3,6];Wang, Qiyao[1,2,3,5,6];Ma, Yue[1,2,3,6];Shao, Shuai[1,2,3,6]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Yantai Marine Econ Res Inst, Yantai 264003, Peoples R China;[5]Shanghai Haosi Marine Biotechnol Co Ltd, Shanghai, Peoples R China;[6]Lab Aquat Anim Dis MOA, Shanghai, Peoples R China

年份：2024

卷号：284

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20241715959714);WOS:【SCI-EXPANDED(收录号:WOS:001298899000001)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (32130108) , the National Key Research and Development Program (2022YFE0101200) , the Shanghai Municipal Science and Technology Commission Technical Leader Project (21XD1431900) , and the China Agriculture Research System of MOF and MARA (CARS-47) .

语种：英文

外文关键词：Aeromonas veronii; Antibiotic resistance; Genome sequencing; Oxidative tolerance; Transposon insertion sequencing

摘要：Aeromonas veronii, , a significant pathogen in aquatic environments, poses a substantial threat to both human and animal health, particularly in aquaculture. In this study, we isolated A. veronii strain GD2019 from diseased largemouth bass ( Micropterus salmoides) ) during a severe outbreak of aeromonad septicemia in Guangdong Province, China. The complete genome sequence of A. veronii GD2019 revealed that GD2019 contains a single chromosome of 4703,168 bp with an average G+C +C content of 58.3 %. Phylogenetic analyses indicated that GD2019 forms a separate sub-branch in A. veronii and comparative genomic analyses identified the existence of an intact Type III secretion system. Moreover, to investigate the genes that are required for the conditional fitness of A. veronii under various stresses, a high-density transposon insertion library in GD2019 was generated by a Tn5-based transposon and covers 6311 genomic loci including 4155 genes and 2156 intergenic regions. Leveraging this library, 630 genes were classified as essential genes for growth in rich-nutrient LB medium. Furthermore, the genes GE001863/NtrC and GE002550 were found to confer tolerance to sodium hypochlorite in A. veronii. . GE002562 and GE002614 were associated with the resistance to carbenicillin. Collectively, our results provide abundant genetic information on A. veronii, , shedding light on the pathogenetic mechanisms of Aeromonas. .