文献类型：期刊文献

英文题名：Identity and public health potential of Cryptosporidium spp. in water buffalo calves in Egypt

作者：Amer, Said[1,2];Zidan, Shereif[3];Feng, Yaoyu[4];Adamu, Haileeyesus[1,5];Li, Na[1,4];Xiao, Lihua[1]

机构：[1]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30329 USA;[2]Kafr El Sheikh Univ, Fac Sci, Dept Zool, Kafe El Sheikh, Egypt;[3]Menoufia Univ, Sadat Branch, Dept Anim Hyg & Zoonoses, Fac Vet Med, Al Menofia, Egypt;[4]E China Univ Sci & Technol, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China;[5]Univ Addis Ababa, Addis Ababa, Ethiopia

年份：2013

卷号：191

期号：1-2

起止页码：123

外文期刊名：VETERINARY PARASITOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000312355000019)】；

基金：This study was supported in part by the Arab Fund for Economic & Social Development "Zamalat Program" and the National Natural Science Foundation of China (Project No. 31110103901 and 31229005). We thank the Kafr El Sheikh University and Menofia University for supporting the field work in Egypt, Centers for Disease Control and Prevention for supporting the molecular analysis, and Dawn Roellig and Hongwei Zhang for technical assistance.

语种：英文

外文关键词：Cryptosporidium; Genotype; Subtype; Buffalo; Calves; Egypt

摘要：Little is known about the diversity and public health significance of Cryptosporidium species in water buffaloes. In this study, we examined the distribution of Cryptosporidium spp. in water buffalo calves in Egypt. Rectal fecal specimens from 179 calves and 359 adults were screened microscopically for Cryptosporidium oocysts using modified Ziehl-Neelsen stain. Cry ptosporidium spp. in 17 microscopy-positive specimens from calves were genotyped by DNA sequence analysis of the small-subunit rRNA gene, and Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium ryanae was found in 10 specimens and C. parvum in 7 specimens, with the former belonging to the newly identified C. ryanae buffalo variant and the latter belonging to the subtypes IldA20G1 (in 5 specimens) and IlaA15G1R1 (in 2 specimens). The prevailing occurrence of C. ryanae and the subtype family lid of C. parvum and the absence of C. bovis and C. andersoni represent some features of Cryptosporidium transmission in water buffaloes in Egypt. Published by Elsevier B.V.