文献类型：期刊文献

英文题名：Electrofabrication of functional materials: Chloramine-based antimicrobial film for infectious wound treatment

作者：Qu, Xue[1];Liu, Huan[1];Zhang, Chuchu[1];Lei, Yu[1];Lei, Miao[1];Xu, Miao[4,5];Jin, Dawei[6];Li, Peng[4,5];Yin, Meng[6];Payne, Gregory F.[2,3];Liu, Changsheng[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]Inst Biosyst & Biotechnol Res, 5115 Plant Sci Bldg, College Pk, MD 20742 USA;[3]Fischell Dept Bioengn, 5115 Plant Sci Bldg, College Pk, MD 20742 USA;[4]Nanjing Tech Univ NanjingTech, Natl Synerget Innovat Ctr Adv Mat SICAM, Key Lab Flexible Elect KLOFE, Nanjing 210009, Jiangsu, Peoples R China;[5]Nanjing Tech Univ NanjingTech, Natl Synerget Innovat Ctr Adv Mat SICAM, IAM, Nanjing 210009, Jiangsu, Peoples R China;[6]Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Dept Cardiothorac Surg, 1678 Dongfang Rd, Shanghai 200127, Peoples R China

年份：2018

卷号：73

起止页码：190

外文期刊名：ACTA BIOMATERIALIA

收录：;EI(收录号：20215111339106);WOS:【SCI-EXPANDED(收录号:WOS:000436222600015)】；

基金：The support from the National Natural Science Foundation of China (51621002, 51573047), the 111 project (B14018), the Fundamental Research Funds for the Central Universities (222201717002) and the United States National Science Foundation (CBET-1435957) and Defense Threat Reduction Agency (HDTRA1-13-0037).

语种：英文

外文关键词：Electrofabrication; Chloramine; Antimicrobial; Wound dressing; Chitosan

摘要：Electrical signals can be imposed with exquisite spatiotemporal control and provide exciting opportunities to create structure and confer function. Here, we report the use of electrical signals to program the fabrication of a chloramine wound dressing with high antimicrobial activity. This method involves two electrofabrication steps: (i) a cathodic electrodeposition of an aminopolysaccharide chitosan triggered by a localized region of high pH; and (ii) an anodic chlorination of the deposited film in the presence of chloride. This electrofabrication process is completed within several minutes and the chlorinated chitosan can be peeled from the electrode to yield a free-standing film. The presence of active N-CI species in this electrofabricated film was confirmed with chlorination occurring first on the amine groups and then on the amide groups when large anodic charges were used. Electrofabrication is quantitatively controllable as the cathodic input controls film growth during deposition and the anodic input controls film chlorination. In vitro studies demonstrate that the chlorinated chitosan film has antimicrobial activities that depend on the chlorination degree. In vivo studies with a MRSA infected wound healing model indicate that the chlorinated chitosan film inhibited bacterial growth, induced less inflammation, developed reorganized epithelial and dermis structures, and thus promoted wound healing compared to a bare wound or wound treated with unmodified chitosan. These results demonstrate the fabrication of advanced functional materials (i.e., antimicrobial wound dressings) using controllable electrical signals to both organize structure through non-covalent interactions (i.e., induce chitosan's reversible self-assembly) and to initiate function-conferring covalent modifications (i.e., generate chloramine bonds). Potentially, electrofabrication may provide a simple, low cost and sustainable alternative for materials fabrication. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.