文献类型：期刊文献

英文题名：Piezoresistive MXene/Silk fibroin nanocomposite hydrogel for accelerating bone regeneration by Re-establishing electrical microenvironment

作者：Hu, Zhi-Chao[1];Lu, Jia-Qi[1];Zhang, Tai-Wei[1];Liang, Hai-Feng[1];Yuan, Hao[2];Su, Di-Han[1];Ding, Wang[3];Lian, Rui-Xian[4];Ge, Yu-Xiang[3];Liang, Bing[5];Dong, Jian[1];Zhou, Xiao-Gang[1];Jiang, Li-Bo[1]

机构：[1]Fudan Univ, Zhongshan Hosp, Dept Orthopaed Surg, Shanghai 200032, Peoples R China;[2]Shanghai Univ, Key Lab Adv Display & Syst Applicat, Minist Educ, Shanghai 200072, Peoples R China;[3]Fudan Univ, Minhang Hosp, Dept Orthoped Surg, Shanghai 201100, Peoples R China;[4]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Res Ctr Biomed Mat, Sch Mat Sci & Engn,Minist Educ, Shanghai 200237, Peoples R China;[5]Fudan Univ, Zhongshan Hosp Xiamen, Dept Orthoped Surg, Xiamen 361015, Peoples R China

年份：2023

卷号：22

起止页码：1

外文期刊名：BIOACTIVE MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000888578900001)】；

基金：This work was supported by National Natural Science Foundation of China, China (No. 82272457, 81972508, 82172738) , "Technology Innovation Action Plan" of Science and Technology Commission of Shanghai Municipality, China (21S11902700) , Natural Science Foundation of Shanghai, China (21ZR1412300) , Shanghai Talent Development Fund, China (2020067) , Shanghai "Rising Stars of Medical Talent" Youth Development Program, China (Youth Medical Talents-Specialist Program, [2020] 087) , Shanghai Sailing Program, China (No. 19YF1406800) , Xiamen Medical and Health Guidance Project, China (3502Z20214ZD1078) . 15

语种：英文

外文关键词：Bone regeneration; Electrical microenvironment; Regenerated silk fibroin; MXene; Electrical stimulation

摘要：The electrical microenvironment plays an important role in bone repair. However, the underlying mechanism by which electrical stimulation (ES) promotes bone regeneration remains unclear, limiting the design of bone microenvironment-specific electroactive materials. Herein, by simple co-incubation in aqueous suspensions at physiological temperatures, biocompatible regenerated silk fibroin (RSF) is found to assemble into nanofibrils with a beta-sheet structure on MXene nanosheets, which has been reported to inhibit the restacking and oxidation of MXene. An electroactive hydrogel based on RSF and bioencapsulated MXene is thus prepared to promote effi-cient bone regeneration. This MXene/RSF hydrogel also acts as a piezoresistive pressure transducer, which can potentially be utilized to monitor the electrophysiological microenvironment. RNA sequencing is performed to explore the underlying mechanisms, which can activate Ca2+/CALM signaling in favor of the direct osteogenesis process. ES is found to facilitate indirect osteogenesis by promoting the polarization of M2 macrophages, as well as stimulating the neogenesis and migration of endotheliocytes. Consistent improvements in bone regeneration and angiogenesis are observed with MXene/RSF hydrogels under ES in vivo. Collectively, the MXene/RSF hydrogel provides a distinctive and promising strategy for promoting direct osteogenesis, regulating immune microenvironment and neovascularization under ES, leading to re-establish electrical microenvironment for bone regeneration.