文献类型：期刊文献

英文题名：Investigation of the immunosuppressive activity of Physalin H on T lymphocytes

作者：Yu, Youjun[1];Sun, Lijuan[1];Ma, Lei[1];Li, Jiyu[3];Hu, Lihong[1,2];Liu, Jianwen[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Gen Surg, Shanghai 200092, Peoples R China

年份：2010

卷号：10

期号：3

起止页码：290

外文期刊名：INTERNATIONAL IMMUNOPHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000275925700005)】；

基金：This work has been supported by the National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204), the 111 Project (No. B07023), Shanghai Leading Academic Discipline Project (No. B507), National Nature Science Foundation of China-Project for Young Scientists Fund (No. 30600598) and the Project of Shanghai Science Committee for Young Scientists (No. 06QA14034). The author would also like to thank some fellow members named Xingming Fan and Junyi Li for completing the research.

语种：英文

外文关键词：Physalin H; Immunosuppressive; Cell mitosis cycle; Delayed-type hypersensitivity; Th1/Th2 balance; Heme oxygenase-1

摘要：Physalis angulata is an annual herb widely used in folk medicine. It is mainly used for treating rheumatoid arthritis (RA). Following bioactivity-guided isolation, a representative immunosuppressive compound, Physalin H was been identified from this herb medicine. The purpose of this work was to assess the immunosuppressive activity of Physalin H on T cells and to explore its potential mode of action. The results showed that Physalin H in a dose-dependent manner significantly inhibited the proliferation of T cells induced by concanavalin A (ConA) and by the mixed lymphocyte culture reaction (MLR). This inhibitive activity was mainly due to interfering DNA replication in G1 stages. In vivo experiments showed that, administration of Physalin H dose-dependently suppressed CD4(+) T cell mediated delayed-type hypersensitivity (DTH) reactions, and suppressed antigen-specific T-cell response in ovalbumin (OVA) immunized mice. Further study indicated that Physalin H could modulate Th1/Th2 cytokine balance and induce the production of immune regulation target Heme oxygenase (HO)-1 in T-cells in vitro. In this study, we demonstrated the immunosuppressive effect of Physalin H on T cells both in vitro and in vivo, and the immunosuppressive activity might be attributed to the suppression of T cell activation and proliferation, the modulation of Th1/Th2 cytokine balance and the induction of HO-1 in T cells. (C) 2009 Elsevier B.V. All rights reserved.