文献类型：期刊文献

英文题名：DNA Cleavage System of Nanosized Graphene Oxide Sheets and Copper Ions

作者：Ren, Hongliu[2];Wang, Chong[2];Zhang, Jiali[1];Zhou, Xuejiao[1];Xu, Dafeng[2];Zheng, Jing[2];Guo, Shouwu[1];Zhang, Jingyan[2]

机构：[1]Shanghai Jiao Tong Univ, Natl Key Lab Micro Nano Fabricat Technol, Key Lab Thin Film & Microfabricat, Minist Educ,Res Inst Micro Nano Sci & Technol, Shanghai 200240, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Pharm, Shanghai 200237, Peoples R China

年份：2010

卷号：4

期号：12

起止页码：7169

外文期刊名：ACS NANO

收录：;EI(收录号：20110113546750);WOS:【SCI-EXPANDED(收录号:WOS:000285449100017)】；

基金：This work was supported by the State key laboratory of bioreactor engineering (No. 2060204), 111 Project (B07023), the National "973 Program" (2007CB936000, 2010CB933900), and the NSFC (Nos. 20774029, 90923041).

语种：英文

外文关键词：graphene oxide; DNA Intercalation; DNA cleavage; copper ion; metal ions

摘要：The exploration of efficient DNA intercalative agents (intercalators) is essential for understanding DNA scission, repair, and signal transduction. In this work, we explored systematically the graphene oxide (GO) interaction with DNA molecules using fluorescence spectroscopic (FL) and circular dichroism (CD) studies, gel electrophoresis, and DNA thermal denaturation. We demonstrated that the GO nanosheets could intercalate efficiently into DNA molecules. Significantly, we illustrated that the scission of DNA by GO sheets combining with copper ions could take place pronouncedly. The scission of DNA by the GO/Cu2+ system is critically dependent on the concentrations of GO and Cu2+ and their ratio. DNA cleavage ability exhibited by the GO with several other metal ions and the fact that GO/Cu2+-cleaved DNA fragments can be partially relegated suggest that the mechanism of DNA cleavage by the GO/metal ion system is oxidative and hydrolytic. The result reveals that the GO/ Cu2+ could be used as a DNA cleaving system that should find many practical applications in biotechnology and as therapeutic agents.