文献类型：期刊文献

英文题名：Ultra-low noise measurements of nanopore-based single molecular detection

作者：Gu, Zhen[1,2];Wang, Huifeng[3];Ying, Yi-Lun[1,2];Long, Yi-Tao[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Informat Sci & Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：62

期号：18

起止页码：1245

外文期刊名：SCIENCE BULLETIN

收录：;EI(收录号：20174004225045);WOS:【SCI-EXPANDED(收录号:WOS:000412131000007)】；

基金：This work was supported by the National Natural Science Foundation of China (21327807, 21421004), Innovation Program of Shanghai Municipal Education Commission (2017-01-07-00-02-E00023) and the Fundamental Research Funds for the Central Universities (222201718001, 222201717003).

语种：英文

外文关键词：Nanopore; Membrane capacitance; Current amplifier; Signal amplifier and recording; High current resolution

摘要：Nanopore is an ultra-sensitive electrochemical technique for single molecular detection in confined space. To suppress the noise in detection of the weak current of nanopore, we investigated the influence of membrane capacitance and applied voltage on the noise of the current signal by model analysis, simulation and experiment. The obtained results demonstrated that membrane capacitance affects the noise by amplifying the noise of the applied voltage. Therefore, suppression of applied voltage noise is an efficient approach for reducing the noise in nanopore detection. Here, we developed an ultra-low noise instrument system for detecting the single molecule signal in nanopores. As demonstrated by nanopore experiments, the p-p noise of the developed system during the recording is reduced to 3.26 pA using the filter of 5 kHz. Therefore, the developed system could be applied in highly sensitive nanopore detection. (C) 2017 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.