文献类型：期刊文献

英文题名：Cost-efficient and process-efficient separation of geniposide from Gardenia jasminoides Ellis by high-performance counter-current chromatography

作者：Zhang, Min[2];Ignatova, Svetlana[1];Hu, Ping[3];Liang, Qionglin[4];Wang, Yiming[4];Sutherland, Ian[1];Jun, Frank Wu[5];Luo, Guoan[2,4]

机构：[1]Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England;[2]E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr Tradit Chinese Med, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]Tsinghua Univ, Inst Biomed, Beijing 100084, Peoples R China;[5]XiangCam TCM Res Ctr Ltd, Unit 16, Meditrina, Cambridge CB22 3AT, England

年份：2012

卷号：89

起止页码：193

外文期刊名：SEPARATION AND PURIFICATION TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000302832800027)】；

基金：This study was financially supported by the National Natural Science Foundation of China (No. 30860381, 21006023) and Shanghai-Unilever Research & Development Fund (No. 08520750300). Further support from Xiang Xue Pharmaceutical, Guanzhou and the Higher Education Funding Council (HEIF4) is gratefully acknowledged.

语种：英文

外文关键词：Counter-current chromatography; Geniposide; Gardenia jasminoides Ellis; Process efficiency; Cost efficiency

摘要：Gardenia jasminoides Ellis has been used for traditional Chinese medicine to treat all forms of febrile diseases. A cost-efficient and process-efficient separation of the bioactive molecule geniposide from G. jasminoides Ellis by high-performance counter-current chromatography (HPCCC) has been developed. Separation by preparative HPCCC was performed with a two-phase solvent system composed of ethyl acetate-butanol-water (0.4:1.6:2, v/v). The separation yielded 445 mg of geniposide with a purity of 95.2% from 4.0 g of crude extract. A factor of cost efficiency was developed and usec. for an economic evaluation of the separation process. The cost efficiency for geniposide was 0.070 g h(-1) $(-1). The separation process by HPCCC was demonstrated to be more economical and efficient than those methods by HSCCC and LC. (c) 2012 Elsevier B.V. All rights reserved.