文献类型：期刊文献

中文题名：摇瓶发酵参数的测定及其应用的探索

英文题名：DETERMINATION OF FERMENTATION PARAMETERS FOR SCAL UP USING A SPECIALLY DESIGNED SHAKE FLASK

作者：范代娣[1];俞俊棠[1]

机构：[1]西北大学化工系,华东理工大学生工所生物反应器国家重点实验室

年份：1996

卷号：12

期号：1

起止页码：91

中文期刊名：化学反应工程与工艺

外文期刊名：Chemical Reaction Engineering and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；

基金：国家教委博士点基金;陕西省教委专项基金

语种：中文

中文关键词：摇瓶;在线检测;溶解氧;发酵参数;气相氧

外文关键词：Shake flask, Ferment. eng. parameters, On-line detection, Ferment. scaleup

摘要：研究制作了一种可在线检测发酵过程中气相氧、溶解氧及中间补料、采样的特殊摇瓶，取得了摇瓶规模下的菌体摄氧率、氧传递系数等重要工程参数，并以摇瓶所得摄氧率（ＯＵＲ）为依据进行发酵放大，通过质谱仪在线检测，验证发酵罐发酵与摇瓶发酵所得数据的异同。
Some important ferment. eng. paramenters, such as: oxygen uptake rate and oxygen transfer coefficient, etc. , were obtained from a specially designed shake flask, in which the gasphase oxygen and liq-phase oxygen could be on-line detected in the ferment. course, based on the permeability P_m determined by the method of sulfite oxidation. Ferment. scale-up were carried on the basis of oxygen uptake rate obtained from the shake flask, through mass spectrometer on-line analysis, a comparison of ferment. parameters obtained between fermentor and shake flask was made.