文献类型：期刊文献

英文题名：Microenvironment-Triggered Signal Amplification for Dual-Modal Fluorescence/MRI of Hepatocellular Carcinoma

作者：Liu, Jihong[1];Xu, Shiyun[1];Yang, Ting[1];Hu, Shanshan[1];Yao, Yongkang[1];Liu, Shichang[1];Tang, Wenjun[1];Ma, Xiaoyu[1];Yang, Haojian[1];Shi, Yiqi[1];Zhao, Weijun[1];Wang, Qi[1,2];Zhu, Wei-Hong[1,2]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Inst Fine Chem,Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Ctr Photosensit Chem Engn, State Key Lab Green Chem Engn & Ind Catalysis, Shanghai 200237, Peoples R China

年份：2026

卷号：20

期号：9

起止页码：7752

外文期刊名：ACS NANO

收录：;EI(收录号：20261120241694);WOS:【SCI-EXPANDED(收录号:WOS:001697620000001)】；

基金：This work was supported by the National Key Research and Development Program of China (2024YFA1803501 and 2021YFA0910000), NSFC (T2488302, 22222803, and 22404056), Science and Technology Commission of Shanghai Municipality (24DX1400200), Fellowship of China National Postdoctoral Program for Innovative Talents (BX20240114), Shanghai Postdoctoral Excellence Program (2024776), and China Postdoctoral Science Foundation (2025M771000).

语种：英文

外文关键词：dual-modal; fluorescence; magnetic resonanceimaging; hepatocellular carcinoma; synergistic amplification

摘要：Dual-modal fluorescence/magnetic resonance imaging (FL/MRI) offers sensitive and high-resolution detection of hepatocellular carcinoma (HCC), but its effectiveness is often limited by weak signal synergy and poor responsiveness to the tumor microenvironment. Here, we report a tumor-microenvironment-responsive nanoprobe, TCM-Gd-P NPs, designed to remain initially signal-silent, as amphiphilic polymer encapsulation sequesters the aggregation-induced emission luminogen (TCM-4COOLi) and restricts water interaction with Gd3+ ions, effectively quenching fluorescence and reducing longitudinal relaxivity. Under acidic tumor conditions, protonation of the polymer matrix restricts intramolecular motion of TCM-4COOLi, triggering up to a 12.4-fold fluorescence enhancement at 584 nm, while TCM-4COOGd complexes increase water proton accessibility, producing an similar to 8.0-fold increase in longitudinal relaxivity and spatially coupled MRI signal amplification. In vivo, TCM-Gd-P NPs achieved 4.2-fold fluorescence and 3.3-fold MRI contrast enhancement in tumors relative to adjacent tissue, enabling molecular-level delineation of tumor margins via fluorescence imaging and high-resolution anatomical mapping via MRI. Importantly, by applying both modalities to the same orthotopic HCC mice, the high sensitivity of fluorescence imaging effectively corroborated the deep-tissue anatomical resolution provided by MRI. This work establishes a microenvironment-triggered, orthogonal signal amplification strategy for FL/MRI, providing a generalizable framework for intelligent solid-tumor diagnostics.