文献类型：期刊文献

中文题名：Cagelike mesoporous silica encapsulated with microcapsules for immobilized laccase and 2,4-DCP degradation

英文题名：Cagelike mesoporous silica encapsulated with microcapsules for immobilized laccase and 2,4-DCP degradation

作者：Junya Yang[1];Yan Huang[1];Yuxiang Yang[1];Hongming Yuan[2];Xiangnong Liu[3]

机构：[1]School of Chemistry and Molecular Engineering,East China University of Science & Technology;[2]State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,College of Chemistry,Jilin University;[3]Analysis Test Center,Yangzhou University

年份：2015

卷号：27

期号：12

起止页码：52

中文期刊名：Journal of Environmental Sciences

外文期刊名：环境科学学报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2015_2016】；PubMed;

基金：supported by the National Natural Science Foundation of China (20577010,20971043);the Open Project Program of State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,Jilin University (2011-30)

语种：英文

中文关键词：Laccase-cMSS ;mLbL-laccase ;2,4-Dichlorophenol; Degradation efficiency ;Reusability ;Degradation mechanism

外文关键词：Laccase-cMSS ;mLbL-laccase ;2,4-Dichlorophenol; Degradation efficiency ;Reusability ;Degradation mechanism

摘要：In this study,cage-like mesoporous silica was used as the carrier to immobilize laccase by a physical approach,followed by encapsulating with chitosan/alginate microcapsule membranes to form microcapsules of immobilized laccase based on layer-by-layer technology.The relationship between laccase activity recovery/leakage rate and the coating thickness was simultaneously investigated.Because the microcapsule layers have a substantial network of pores,they act as semipermeable membranes,while the laccase immobilized inside the microcapsules acts as a processing plant for degradation of2,4-dichlorophenol.The microcapsules of immobilized laccase were able to degrade 2,4-dichlorophenol within a wide range of 2,4-dichlorophenol concentration,temperature and p H,with mean degradation rate around 62%.Under the optimal conditions,the thermal stability and reusability of immobilized laccase were shown to be improved significantly,as the removal rate and degradation rate remained over 40.2% and 33.8% respectively after 6 cycles of operation.Using mass spectrometry（MS） and nuclear magnetic resonance（NMR）,diisobutyl phthalate and dibutyl phthalate were identified as the products of 2,4-dichlorophenol degradation by the microcapsules of immobilized laccase and laccase immobilized by a physical approach,respectively,further demonstrating the degradation mechanism of 2,4-dichlorophenol by microcapsule-immobilized laccase.
In this study,cage-like mesoporous silica was used as the carrier to immobilize laccase by a physical approach,followed by encapsulating with chitosan/alginate microcapsule membranes to form microcapsules of immobilized laccase based on layer-by-layer technology.The relationship between laccase activity recovery/leakage rate and the coating thickness was simultaneously investigated.Because the microcapsule layers have a substantial network of pores,they act as semipermeable membranes,while the laccase immobilized inside the microcapsules acts as a processing plant for degradation of2,4-dichlorophenol.The microcapsules of immobilized laccase were able to degrade 2,4-dichlorophenol within a wide range of 2,4-dichlorophenol concentration,temperature and p H,with mean degradation rate around 62%.Under the optimal conditions,the thermal stability and reusability of immobilized laccase were shown to be improved significantly,as the removal rate and degradation rate remained over 40.2% and 33.8% respectively after 6 cycles of operation.Using mass spectrometry（MS） and nuclear magnetic resonance（NMR）,diisobutyl phthalate and dibutyl phthalate were identified as the products of 2,4-dichlorophenol degradation by the microcapsules of immobilized laccase and laccase immobilized by a physical approach,respectively,further demonstrating the degradation mechanism of 2,4-dichlorophenol by microcapsule-immobilized laccase.