文献类型：期刊文献

英文题名：Treatment of Denervated Muscle Atrophy by Injectable Dual-Responsive Hydrogels Loaded with Extracellular Vesicles

作者：Bu, Ziheng[1];Jing, Jianxing[2];Liu, Wei[1];Fan, Zhen[2];Huang, Junchao[1];Zhou, Zheng[1];Hu, Jianhai[1];An, Jinxi[3];Hong, Jiachang[1];Yu, Jianing[3];Tang, Daolin[4];Sun, Min[1,5];Du, Jianzhong[1,2,5];Wu, Peng[1]

机构：[1]Tongji Univ, Shanghai Peoples Hosp 10, Dept Orthoped, Sch Med, Shanghai 200072, Peoples R China;[2]Tongji Univ, Sch Mat Sci & Engn, Dept Polymer Mat, 4800 Caoan Rd, Shanghai 201804, Peoples R China;[3]Anhui Univ Sci & Technol, Sch Med, 168 Taifeng St, Huainan 232000, Anhui, Peoples R China;[4]UT Southwestern Med Ctr, Dept Surg, Dallas, TX 75390 USA;[5]East China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2025

卷号：12

期号：10

外文期刊名：ADVANCED SCIENCE

收录：;EI(收录号：20250417743757);WOS:【SCI-EXPANDED(收录号:WOS:001400921000001)】；

基金：Z.B., J.J., and W.L. contributed equally to this work. This research was supported by National Natural Science Foundation of China (52222306, 22075212, 21925505 and 22305177), the International Scientific Collaboration fund of Science and Technology Commission of Shanghai Municipality (21520710100, 23520710900), the fellowship of China Postdoctoral Science Foundation (2022M720107, GZB20230517) and Shanghai "Super Postdoc" Incentive Plan (2022568), Shanghai Rising-Star Program (Sailing, 23YF1433000), Shanghai Municipal Health Commission Clinical Research General Project Fund (03.02.22.007), Shanghai Tenth People's Hospital National Natural Science Foundation Internal Incubation Project Fund (04.03.19.122). J.D. is the recipient of National Science Fund for Distinguished Young Scholars.

语种：英文

外文关键词：denervated muscle atrophy; extracellular vesicles; hydrogel; ultrasound responsiveness

摘要：Denervated muscle atrophy, a common outcome of nerve injury, often results in irreversible fibrosis due to the limited effectiveness of current therapeutic interventions. While extracellular vesicles (EVs) offer promise for treating muscle atrophy, their therapeutic potential is hindered by challenges in delivery and bioactivity within the complex microenvironment of the injury site. To address this issue, an injectable hydrogel is developed that is responsive to both ultrasound and pH, with inherent anti-inflammatory and antioxidant properties, designed to improve the targeted delivery of stem cell-derived EVs. This hydrogel system allows for controlled release of EVs from human umbilical cord mesenchymal stem cells (HUC-MSCs), adapting to the specific conditions of the injury environment. In vivo studies using a rat model of nerve injury demonstrated that the EV-loaded hydrogel (EVs@UR-gel) significantly preserved muscle function. Six weeks post-nerve reconstruction, treated rats exhibited muscle strength, circumference, and wet weight reaching 89.53 +/- 0.96%, 76.02 +/- 7.49%, and 88.0 +/- 2.65% of healthy controls, respectively, alongside an improvement in the sciatic nerve index (-0.11 +/- 0.09). This platform presents a novel therapeutic approach by maintaining EV bioactivity, enabling tunable release based on the disease state, and facilitating the restoration of muscle structure and function.