文献类型：期刊文献

英文题名：Analysis of the mechanism of exogenous indole-3-acetic acid on the enrichment of D-glucose in Chlorococcum humicola cultured by sludge extracts

作者：Yu, Xiao[1];Zhao, Jiamin[1];Song, Meijing[1];Li, Renjie[1];Yang, Yingying[1];Ye, Xiaoyun[1];Chen, Xiurong[1,2]

机构：[1]East China Univ Sci & Technol, Natl Engn Res Ctr Ind Wastewater Detoxicat & Resou, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asses, Shanghai 200237, Peoples R China

年份：2023

卷号：902

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20233314546319);WOS:【SCI-EXPANDED(收录号:WOS:001057855500001)】；

基金：Acknowledgments This research was supported by the National Natural Science Foun-dation (No. 51878278) .

语种：英文

外文关键词：Chlorococcum humicola; Indole-3-acetic acid; Polysaccharide accumulation; Proteomics; D-glucose enrichment

摘要：Addressing problems of high organic toxicity in the wastewater treatment process, microalgae have been used to reduce the toxicity in sludge and to synthesize non-toxic and recoverable biomass of resources. Phytohormone is a core regulator of plant growth and current research has generally focused on their promotion of cell division and cell expansion. Effects of phytohormone on the enrichment mechanism of microalgae directional polysaccharides accumulation remain poorly elucidated. This study was carried out to investigate the effects of exogenous indole-3-acetic acid (IAA) on growth characteristics, biomass accumulation, and photosynthesis capacity of Chlorococcum humicola cultured in sludge extract and further find the D-glucose enrichment mechanism of it through proteomic. The results indicated that the optimal culture conditions were the 75 % sludge extract and 25 % selenite enrichment (SE) medium with 5 x 10-6 mol/L indole-3-acetic acid. Polysaccharides increased significantly from day 20 and accumulated to (326.59 & PLUSMN; 13.06) mg/L on day 30, in which the D-glucose proportion increased to 61.53 %. Most notably, proteomic tests were performed and found that the photosynthesis related proteins including the differential proteins of photosystem electron transport, ATP and NADPH catalytic synthesis were significantly up-regulated. At the end of the path, three pathways of D-glucose enrichment with & alpha;-D-Glucose-1P as a precursor were summarized through indole-3-acetic acid activation on amylase, endoglucanase and Beta-glucosidase, etc. These results provide insights to explore the directed enrichment of biomass in Chlorococcum humicola by indole-3-acetic acid.