文献类型：期刊文献

英文题名：MiR-146a suppresses hepatocellular carcinoma by downregulating TRAF6

作者：Zu, Yong[1];Yang, Yanhong[1];Zhu, Jiyun[2];Bo, Xiaobo[3];Hou, Shuangxing[4];Zhang, Bo[3];Qiu, Jiangfeng[2];Zheng, Jing[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, 130 Meilong Rd, Shanghai, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Dept Gastrointestinal Surg, 160 Pujian Rd, Shanghai, Peoples R China;[3]Fu Dan Univ, Zhong Shan Hosp, Dept Gen Surg, 180 Feng Lin Rd, Shanghai, Peoples R China;[4]Fudan Univ, Pudong Med Ctr, Shanghai Pudong Hosp, Dept Neurol, 2800 Gongwei Rd, Shanghai, Peoples R China

年份：2016

卷号：6

期号：11

起止页码：2502

外文期刊名：AMERICAN JOURNAL OF CANCER RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000390591500008)】；

基金：This work was supported by the Shanghai Key Laboratory of New Drug Design via Shanghai Natural Science Foundation (15ZR1409700), and National Natural Science Foundation of China Youth Found (31000474). We thank Prof. Jin Huang for her insightful discussions and Dr. Yan Mei for his help with manuscript preparation.

语种：英文

外文关键词：Hepatocellular carcinoma; miR-146a; proliferation; invasion; TRAF6; xenografted tumor

摘要：MicroRNAs have been proven to play important roles in many biological processes such as cellular growth and differentiation, apoptosis, and modulation of host response to viral infection. In the present study, we find that the expression of miR-146a was decreased in hepatocellular carcinoma (HCC) tissues compared with corresponding adjacent tissues, and the expression level in HCC cell lines was lower than in a normal liver cell. Over-expression suppressed the proliferation and invasion of HCC cells. In addition, luciferase reporter assays and western blotting confirmed that miR-146a directly target TRAF6 which attenuated the effect of miR-146a on cell proliferation and invasion in HepG2 and SMMC7721 cells. Meanwhile, lentivirus-mediated increased expression of miR146a repressed tumor formation in nude mice. Taken together, our findings demonstrate that miR-146a suppresses HCC by down-regulating TRAF6. We also discovered that miR-146a may represent a novel potential candidate of the HCC carcinoma diagnostic marker in the long term.