文献类型：期刊文献

英文题名：Biosynthesis of L-Erythrose by Assembly of Two Key Enzymes in Gluconobacter oxydans

作者：Zou, Xingxing[1];Lin, Jinping[1];Mao, Xinlei[1];Zhao, Shengyun[2];Ren, Yuhong[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Wuyi Univ, Fujian Key Lab Ecoind Green Technol, Wuyishan 354300, Peoples R China

年份：2017

卷号：65

期号：35

起止页码：7721

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20173704155616);WOS:【SCI-EXPANDED(收录号:WOS:000410255400015)】；

基金：This work was funded by the National Special Fund for the State Key Laboratory of Bioreactor Engineering (Grant 2060204), and The National Natural Foundation of China (Grant 21778018).

语种：英文

外文关键词：L-erythrose; self-assembly; G. oxydans; protein peptide interactions

摘要：L-erythrose, a rare aldotetrose, possesses various pharmacological activities. However, efficient L-erythrose production is challenging. Currently, L-erythrose is produced by a two-step fermentation process from erythritol. Here, we describe a novel strategy for the production of L-erythrose in Gluconobacter oxydans (G. oxydans) by localizing the assembly of L-ribose isomerase (L-RI) to membrane-bound sorbitol dehydrogenase (SDH) via the protein peptide interactions of the PDZ domain and PDZ ligand. To demonstrate this self-assembly, green fluorescent protein (GFP) replaced L-RI and its movement to membrane-bound SDH was observed by fluorescence microscopy. The final L-erythrose production was improved to 23.5 g/L with the stepwise metabolic engineering of G. oxydans, which was 1.4-fold higher than that obtained using coexpression of SDH and L-RI in G. oxydans. This self-assembly strategy shows remarkable potential for farther improvement of L-erythrose production.