文献类型：期刊文献

英文题名：Simulation of in situ oil reservoir conditions in a laboratory bioreactor testing for methanogenic conversion of crude oil and analysis of the microbial community

作者：Xu, Dan[1]; Zhang, Kai[1]; Li, Bai-Guang[3]; Mbadinga, Serge Maurice[1]; Zhou, Lei[1]; Liu, Jin-Feng[1]; Yang, Shi-Zhong[1]; Gu, Ji-Dong[2]; Mu, Bo-Zhong[1,4]

机构：[1] State Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [2] School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, Hong Kong; [3] The Second Oil Production Factory of Daqing Oilfield Company, CNPC, Daqing, Heilongjiang Province, 163414, China; [4] Engineering Research Center of MEOR, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

年份：2019

卷号：136

起止页码：24

外文期刊名：International Biodeterioration and Biodegradation

收录：EI(收录号：20184305983054)

语种：英文

外文关键词：Crude oil - RNA - Fatty acids - Methane - Petroleum reservoirs - Bacteria - Enhanced recovery - Petroleum reservoir engineering - Methanogens - Biodegradation

摘要：A bioreactor was designed and established to simulate in situ oil reservoir conditions for investigating methanogenic conversion of crude oil by microbial community derived from production water of a high-temperature oilfield. The continuous incubation and culturing were divided into two phases (Phase I, day 0–341; and Phase II, day 341–520) to allow sampling and supplement of fresh culture medium on day 341 and 520, respectively. The average methane production rate was 4.9 and 20.8 mmol CH4·day?1 m?3 pore volume during the Phase I and Phase II, respectively. Degradation intermediates of fatty acids were detected in the culture fluids indicating the transformation of crude oil. The 16S rRNA gene sequence analysis revealed that the major bacterial community shifted from Deferribacteres to Aminicenantes and unclassified Bacteria, as well as Thermotogae and Synergistetes. The predominant methanogenic community shifted from methylotrophic methanogen (Methanolobus) to hydrogenotrophic methanogens (Methanothermobacter and Methanocalculus) and acetoclastic methanogen (Methanothrix) after 341 days of incubation, and was subsequently dominated by hydrogenotrophic methanogen (Methanothermobacter) after 520 days of incubation. This study expanded our understandings of methanogenic oil-degrading processes and the key microorganisms involved in laboratory simulation bioreactor over time of incubation. In addition, it further provides valuable insights on the microbial conversion of hydrocarbons to methane for enhancing the energy recovery from residual oil in petroleum reservoirs. ? 2018