文献类型：期刊文献

英文题名：Synergistic protective effect of picroside II and NGF on PC12 cells against oxidative stress induced by H₂O₂

作者：Liu, Jian-Wen[1];Yu, You-Jun[1];Zheng, Pei-yong[2];Zhang, Xiao-dong[1];Li, Ting[1];Cao, Yan[1];Guo, Ming-chuan[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Pharm, Shanghai 200237, Peoples R China;[2]Shanghai Tradit Chinese Med Univ, Longhua Hosp, Shanghai 200032, Peoples R China

年份：2007

卷号：59

期号：5

起止页码：573

外文期刊名：PHARMACOLOGICAL REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000251789400010)】；

语种：英文

外文关键词：hydrogen peroxide; nerve growth factor; PC12 cells; picroside II; reactive oxygen species

摘要：Epidemiological studies suggest that nerve growth factor (NGF) is associated with a reduced risk of acute or chronic neuropathies. We studied the synergistic protective effect of picroside II and NGF against the oxidative stress, in PC 12 cells induced by hydrogen peroxide (H2O2). The fluorescent probe CDCFH was used to assess the intracellular reactive oxygen species (ROS) level, and MTT assay, morphological observation as well as LDH leakage test were conducted to measure cellular injury. The H2O2-induced cytotoxicity was significantly attenuated in the presence of picroside H (25 mu g/ml) and NGF (2 ng/ml). Cultures with this combined treatment possessed decreased level of ROS while increased cell survival, as compared to that of picroside H or NGF alone-treated cells. Accordingly, it was concluded that their synergistic protective activities against oxidative stress in vitro were demonstrated in various aspects, including reversing morphological changes, enhancing the ability of cell proliferation and ROS scavenging. Such action supports the therapeutic potential of picroside II and NGF in treating nervous disorders based on their synergistic effect.