文献类型：期刊文献

英文题名：Chlorophyll as key indicator to evaluate astaxanthin accumulation ability of Haematococcus pluvialis

作者：Fang, Lei[1];Zhang, Jingkui[1];Fei, Zhongnan[1];Wan, Minxi[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Meilong Rd 130,Mail Box 301, Shanghai 200237, Peoples R China

年份：2019

卷号：6

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513084574);WOS:【SCI-EXPANDED(收录号:WOS:000511685600001)】；

基金：This research was funded by National Natural Science Foundation of China (31500062), China Postdoctoral Science Foundation (2014T70400), and Fundamental Research Funds for the Central Universities (222201414024).

语种：英文

外文关键词：Astaxanthin; Chlorophyll; Haematococcus pluvialis; Metabolic network; Transcriptome analysis

摘要：Background Natural astaxanthin is mainly derived from Haematococcus pluvialis. In the photoinduction phase, astaxanthin accumulation ability can be significantly affected by the characteristics of H. pluvialis cells in the proliferation phase. Based on sequential heterotrophy-dilution-photoinduction (SHDP) technology, the authors' previous study showed that high astaxanthin accumulation ability is accompanied by high chlorophyll content of H. pluvialis heterotrophic cell; whereas the mechanism of this result remained largely obscure. Therefore, transcriptome analysis was conducted to explain this mechanism. Results RNA-seq analysis showed that the transcription level of chlorophyll synthesis-related genes was negatively correlated with genes related to astaxanthin synthesis. A metabolic network between chlorophyll synthesis and astaxanthin accumulation was proposed. Conclusions The relationship between chlorophyll synthesis and astaxanthin accumulation was clarified. Chlorophyll degradation products might be used for astaxanthin synthesis through certain pathways. This study enlightens on the mechanism for the transformation of pigment and is conducive to optimize culture process of H. pluvialis by improving the chlorophyll content of heterotrophic cell.