文献类型：期刊文献

中文题名：Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

英文题名：Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

作者：Ting Wu[1];Xiao-Hui Yang[1];Chuan-Jing Zhang[1];Zhen-Ping Wang[1];Yi-Ping Du[1]

机构：[1]Shanghai Key Laboratory of Functional Materials Chemistry, Research Centre of Analysis and Test, East China University of Science and Technology

年份：2016

卷号：27

期号：6

起止页码：901

中文期刊名：Chinese Chemical Letters

外文期刊名：中国化学快报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2015_2016】；PubMed;

基金：supported by the National Natural Science Foundation of China (No. 21205041);the Fundamental Research Funds for the Central Universities (No. 222201314039);a grant from Shanghai Municipal Education Committee (No. YJ0114209)

语种：英文

中文关键词：MALDI imaging; Quantitative analysis; Label-free; Microarray; Patterned hydrophilic chip;

外文关键词：MALDI imaging; Quantitative analysis; Label-free; Microarray; Patterned hydrophilic chip;

摘要：Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging（MALDI MSI）.Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/μ L to 1 pmol/μ L was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.
Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging（MALDI MSI）.Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/μ L to 1 pmol/μ L was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.