文献类型：期刊文献

英文题名：Redox-Regulated Phase Switchable Peptide Droplets with Degradation Resistance for Intravenous Delivery of Biopharmaceuticals

作者：Chen, Ran[1];Jiang, Yanan[1];Lv, Mingchen[1];Xu, Jiaxi[1];Liu, Yaping[1];Qin, Jinlong[1];Chen, Zhe-Sheng[3];Sun, Min[2];Fan, Zhen[1];Du, Jianzhong[1,2]

机构：[1]Tongji Univ Mat Sci & Engn, Dept Polymer Mat, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai, Peoples R China;[3]St Johns Univ, Coll Pharm & Hlth Sci, Queens, NY USA

年份：2026

卷号：38

期号：11

外文期刊名：ADVANCED MATERIALS

收录：;EI(收录号：20260219890901);WOS:【SCI-EXPANDED(收录号:WOS:001656307700001)】；

基金：This research was supported by National Natural Science Foundation of China (52222306, 22475154, 22335005, and 22305177), international scientific collaboration fund of Science and Technology Commission of Shanghai Municipality (23520710900), Innovation Program of Shanghai Municipal Education Commission (2023ZKZD28), Shanghai Rising-Star Program (Sailing, 23YF1433000), the Fundamental Research Funds for the Central Universities, Science and Technology Innovation Plan of Shanghai Science and Technology Commission (23520710900). Dr. Erik Jan Cornel (previous PDRA in J. Du group) helped review and revise the entire manuscript.

语种：英文

外文关键词：coacervate; peptide; self-assembly; vesicles

摘要：Liquid-liquid phase separation (LLPS) plays a ubiquitous and vital role during the formation of biomolecular coacervates, and the switchable phase transition between liquid and solid of these coacervates is crucial for a series of dynamic biochemical functions. Currently, tremendous work has been focused on constructing artificial coacervates using synthetic biomolecules; however, such a phase switch has not been achieved. Here, we demonstrate that by designing peptide sequences to match specific in vivo microenvironments, it is practical to utilize redox reactions to control the phase switch of peptide coacervates. Upon optimizing the chemical structure and concentration of peptide, as well as pH and temperature, both phase-separation and phase-transition dynamics could be finely regulated. Cyclically, liquid-like membraneless coacervates are oxidized into solid-like semipermeable coacervate vesicles, and return to liquid-like membraneless coacervates under reductive microenvironments. Furthermore, animal experiments confirmed that oxidized solid-like coacervate vesicles are able to remain stable against degradation during intravenous administration. Upon arriving at the reductive tumor site, solid-like coacervate vesicles gradually become liquid-like coacervate to promote intracellular siRNA delivery with significant inhibition effects against pancreatic tumor, which could pave the way for a new front of manipulating biomolecular LLPS for delivery of biopharmaceuticals.