文献类型：期刊文献

英文题名：Endosomal pH-activatable magnetic nanoparticle-capped mesoporous silica for intracellular controlled release

作者：Gan, Qi[1,2];Lu, Xunyu[1];Dong, Wenjie[1];Yuan, Yuan[1];Qian, Jiangchao[2];Li, Yongsheng[1];Shi, Jianlin[1];Liu, Changsheng[1,2]

机构：[1]E China Univ Chem Technol, Key Lab Ultrafine Mat, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China;[2]E China Univ Chem Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：22

期号：31

起止页码：15960

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY

收录：;EI(收录号：20123015273427);WOS:【SCI-EXPANDED(收录号:WOS:000306479600066)】；

基金：We greatly thank the National Basic Research Program of China (973 Program, no. 2012CB933600), the National Natural Science Foundation of China (no. 31070850 and 31100679), the Shanghai Science and Technology Commission (no. 10540709800) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT0825) for financial support. We also thank Dr Shengnian Wang of Louisiana Tech University for his good advice and English modification.

语种：英文

外文关键词：Nanomagnetics - Cells - Nanoparticles - pH sensors - Silica - Magnetite - Targeted drug delivery - Cell culture - Molecular biology - Disease control - Physiological models

摘要：Endosomal pH-driven linkage-disintegration is a promising strategy to achieve intracellular delivery and controlled drug release. In this paper, a rapid endosomal pH-sensitiveMSNs ensemble (i.e., MCM-TAA-Fe3O4) with magnetic nanoparticle caps was developed by anchoring superparamagnetic Fe3O4 nanoparticles on the pore openings with an acid-labile substituted 1,3,5-triazaadamantane (TAA) group. The functionalized Fe3O4 nanoparticles served as a nanogate to regulate the release pattern and/or dosage of payload. The in vitro release experiment with model dexamethasone showed that the MCM-TAA-Fe3O4 ensembles exhibited quick release at pH 5.0-6.0 and zero release in physiological environment (pH = 7.4). Demonstrated with a MC3T3-E1 model cell line, this hybrid nanomaterial could successfully be endocytosed into cells and then release the encapsulated exogenous cargos into the cytosol. The new rapid endosomal pH-sensitive Fe3O4-capped-MSNs could serve as efficient carriers for intracellular controlled release of therapeutic agents in live cells, and may be potentially applied in clinical disease therapy, especially therapeutics and the metabolic manipulation of cells.