文献类型：期刊文献

英文题名：Analyte Regeneration Fluorescent Probes for Formaldehyde Enabled by Regiospecific Formaldehyde-Induced Intramolecularity

作者：Xu, Hang[1,2];Xu, Huan[1,2];Ma, Shengnan[1,2];Chen, Xiani[1,2];Huang, Lixian[1,2];Chen, Junwei[1,2];Gao, Feng[1,2];Wang, Rui[1,2];Lou, Kaiyan[1,2];Wang, Wei[1,2,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioengn Reactor, Shanghai Key Lab New Drug Design, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Univ Arizona, Dept Pharmacol & Toxicol, Tucson, AZ 85721 USA;[4]Univ Arizona, BIOS Inst, Tucson, AZ 85721 USA

年份：2018

卷号：140

期号：48

起止页码：16408

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20184906208609);WOS:【SCI-EXPANDED(收录号:WOS:000452693800006)】；

基金：The work was supported by the National Natural Science Foundation of China (Grant No. 21577037 to K.L. and 21738002 to W.W.) and the State Key Laboratory of Bioengineering Reactor (W.W.).

语种：英文

外文关键词：Fluorescence spectroscopy - Fluorescence - Probes - Molecules

摘要：An important challenge for reaction-based fluorescent probes is that they generally require analyte consumption for fluorescence signal generation, thus creating potential perturbation of native analyte homeostasis or change of local concentrations. Herein, we reported two formaldehyde (FA) regeneration fluorescent probes, NAP-FAP-1 and NAP-FAP-2. An unprecedented regiospecific FA-induced intramolecularity strategy is implemented in the probe design, which adopts 3-(benzylamino)-succinimide as the FA-selective reaction group. The probes are able to capture the analyte molecule, induce regiospecific imide bond cleavage, and then release the captured FA molecule with simultaneous fluorescence turn-on response via a unique dual PeT/ICT quenching mechanism. The probes have shown potentials in detection, comparison, and imaging of FA levels intracellularly and inside lysosomes. These features make them useful for the study of FA homeostasis and functions in biological systems with minimal perturbation.