文献类型：期刊文献

英文题名：Efficient production of l-menthol in a two-phase system with SDS using an immobilized bacillus subtilis esterase

作者：Pan, Jiang[1]; Dang, Ngoc-Duy[1]; Zheng, Gao-Wei[1]; Cheng, Bo[2]; Ye, Qin[1]; Xu, Jian-He[1]

机构：[1] Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [2] Sunflower Pharmaceutical Group Company Limited, 85 Sihuan Road, Chongqing, 408000, China

年份：2014

卷号：1

期号：1

外文期刊名：Bioresources and Bioprocessing

收录：EI(收录号：20224513086721)

语种：英文

外文关键词：Cloning - Crosslinking - Enantioselectivity - Enzymatic hydrolysis - Enzyme activity - Enzyme inhibition - Esters - Sulfur compounds

摘要：Background: levo-Menthol is an important flavoring chemical, which can be prepared by enantioselective enzymatic hydrolysis of dl-menthyl esters. A recombinant esterase (BsE) cloned from Bacillus subtilis 0554 shows excellent enantioselectivity to dl-menthyl acetate and has been immobilized using cross-linked enzyme aggregates. Though BsE has relatively high substrate tolerance, the conversion of dl-menthyl acetate decreased sharply with the increase of substrate loading from 1 to 3 M in mono-aqueous system, which might be due to the severe inhibition of enzyme activity at extremely high load of substrate or product. In this work, enzymatic hydrolysis of dl-menthyl acetate with an extremely high load using the immobilized CLEA-BsE was investigated in an organic-aqueous biphasic system containing surfactant to establish a promising bioprocess for large-scale production of l-menthol. Results: An efficient biphasic reaction system of pentanol-water containing sodium dodecyl sulfate (SDS) was developed for improving enantioselective hydrolysis of dl-menthyl acetate to produce l-menthol by immobilized BsE. Under the optimized reaction conditions, l-menthol was produced in >97% enantiomeric excess (ee) at a substrate load of up to 3.0 M with >40% conversion. Conclusions: All the positive features demonstrate the potential applicability of the bioprocess for the large-scale production of l-menthol. ? 2014 Pan et al.