文献类型：期刊文献

英文题名：Enhancement of hepatitis B surface antigen production by adenosine 5′-monophosphate in culture of recombinant Chinese hamster ovary cells

作者：Luo, Weiwen[1]; Sun, Xiangming[1]; Yi, Xiaoping[1]; Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2005

卷号：100

期号：4

起止页码：475

外文期刊名：JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录：;EI(收录号：2006069687673);WOS:【SCI-EXPANDED(收录号:WOS:000233831600019)】；

语种：英文

外文关键词：animal cell culture; adenosine 5 '-monophosphate; adenosine 5 '-triphosphate; Chinese hamster ovary (CHO) cell; hepatitis B surface antigen (HBsAg)

摘要：Adenosine 5'-monophosphate was found effective in enhancing hepatitis B surface antigen (HBsAg) production in the culture of recombinant Chinese hamster ovary (CHO) cells, but it markedly inhibited cell growth. A culture strategy with periodical medium changes was established to increase HBsAg production and avoid the AMP-induced inhibition of cell growth. The enhanced energy supply might be one of the reasons for the increase in HBsAg production in the presence of AMP.