文献类型：期刊文献

英文题名：A FRET-enabled molecular peptide beacon with a significant red shift for the ratiometric detection of nucleic acids

作者：Maity, Debabrata[1];Jiang, Juanjuan[2,3];Ehlers, Martin[1];Wu, Junchen[2,3];Schmuck, Carsten[1]

机构：[1]Univ Duisburg Essen, Inst Organ Chem, D-45117 Essen, Germany;[2]E China Univ Sci & Technol, Key Lab Adv Materials, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2016

卷号：52

期号：36

起止页码：6134

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000375346500021)】；

语种：英文

摘要：A cationic molecular peptide beacon NAP1 functionalized with a fluorescence resonance energy transfer-pair at its ends allows the ratiometric detection of ds-DNA with a preference for AT rich sequences. NAP1 most likely binds in a folded form into the minor groove of ds-DNA, which results in a remarkable change in its fluorescence properties. As NAP1 exhibits quite low cytotoxicity, it can also be used for imaging of nuclear DNA in cells.