文献类型：期刊文献

英文题名：Effective pretreatment of sugarcane bagasse with combination pretreatment and its hydrolyzates as reaction media for the biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by whole cells of E-coli CCZU-K14

作者：He, Yu-Cai[1,2,3];Zhang, Dan-Ping[1];Di, Jun-Hua[1];Wu, Yin-Qi[1];Tao, Zhi-Cheng[1];Liu, Feng[1];Zhang, Zhi-Jun[2];Chong, Gang-Gang[1];Ding, Yun[1];Ma, Cui-Luan[1]

机构：[1]Changzhou Univ, Coll Pharmaceut Engn & Life Sci, Biochem Engn Lab, Changzhou, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Washington State Univ, Dept Biol Syst Engn, Bioprod Sci & Engn Lab, Richland, WA 99354 USA

年份：2016

卷号：211

起止页码：720

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20161502233855);WOS:【SCI-EXPANDED(收录号:WOS:000375186700090)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21102011), the Natural Science Foundation of Jiangsu Province (No. BK20141172), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Open Project Program of the State Key Laboratory of Bioreactor Engineering, and the Key Laboratory of Guangxi Biorefinery (No. GXBF11-03).

语种：英文

外文关键词：Sugarcane bagasse; Combination pretreatment; Ethyl 4-chloro-3-oxobutanoate; Ethyl (S)-4-chloro-3-hydroxybutanoate; Asymmetric bioreduction

摘要：In this study, sugarcane bagasse (SB) was pretreated with combination pretreatment (e.g., sequential KOH extraction and ionic liquid soaking, sequential KOH extraction and Fenton soaking, or sequential KOH extraction and glycerol soaking). After the enzymatic hydrolysis of pretreated SBs, it was found that all these three concentrated hydrolyzates could be used for the asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate (COBE) into ethyl (S)-4-chloro-3-hydroxybutanoate [(S)-CHBE]. Compared with glucose, arabinose and cellobiose couldn't promote the initial reaction rate, and xylose could increase the intracellular NADH content. Moreover, it was the first report that hydrolyzates could be used for the effective biosynthesis of (S)-CHBE (similar to 500 g/L; 98.0% yield) from 3000 COBE by whole cells of Escherichia coli CCZU-K14 in the presence of beta-CD (0.4 mol beta-CD/mol COBE), L-glutamine (200 mM) and glycine (500 mM). In conclusion, it is a new alternative to utilize bioresource for the synthesis of key chiral intermediate (S)-CHBE. (C) 2016 Elsevier Ltd. All rights reserved.