文献类型：期刊文献

英文题名：Brocaeloid D, a novel compound isolated from a wheat pathogenic fungus, Microdochium majus 99049

作者：Zhang, Jingyu[1];Wang, Zhenzhen[1];Song, Zhijun[2,5];Karthik, Loganathan[1];Hou, Chengjian[1];Zhu, Guoliang[1];Jiang, Lan[1];Han, Jianying[2,5];Ma, Rong[1];Li, Li[3];Zhang, Lixin[1,2];Liu, Xueting[1];Hsiang, Tom[4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China;[3]Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, Dept Med Chem, Beijing 100050, Peoples R China;[4]Univ Guelph, Sch Environm Sci, 50 Stone Rd East, Guelph, ON N1G 2W1, Canada;[5]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

年份：2019

卷号：4

期号：4

起止页码：173

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000503371600001)】；

基金：This work was partially supported by the grants from the National Natural Science Foundation of China (31430002, 81573341, 21877038, 31720103901, 31320103911), Taishan Scholarship, Open Project Funding of the State Key Laboratory of Bioreactor Engineering, the 111 Project (B18022), National Key R&D Program of China 2017YFE0108200, and the Fundamental Research Funds for the Central Universities (22221818014). Discovery and isolation of Microdochium majus strain 99049 was supported by the Natural Sciences and Engineering Research Council of Canada funding to T. Hsiang.

语种：英文

外文关键词：Wheat pathogenic fungus; Anticancer; Antibacterial; Natural product

摘要：Microbes serve as the most important resource for drug discovery. During our screening for bioactive compounds from our natural products library, a pathogenic fungus, Microdochium majus strain 99049, from wheat was selected for further investigation. A new alkaloid named brocaeloid D (1), together with six previously characterized compounds (2-7) were identified. Compound 1 belongs to 4-oxoquinoline with C-2 reversed prenylation and a succinimide substructure. All the structures of these newly isolated compounds were determined by different means in spectroscopic experiments. The absolute configurations of 1 was further deduced from comparison of its CD spectrum with that of known compound 2. The bioactivities of these identified compounds were evaluated against several pathogenic microorganisms and cancer cell lines. Compounds 1-5 showed activity against HUH-7 human hepatoma cells with IC50 values of 80 mu g/mL. Compound 6 showed mild activity against HeLa cells (IC50= 51.9 mu g/mL), weak anti-MTB activity (MIC = 80 mu g/mL), and moderate anti-MRSA activity (MIC = 25 mu g/mL), and compound 7 showed weak anti-MRSA activity (MIC = 100 mu g/mL).