文献类型：期刊文献

英文题名：Preparation of a low bleeding polar stationary phase for hydrophilic interaction liquid chromatography

作者：Qian, Kun[1];Peng, Yahui[1,2];Zhang, Feifang[1];Yang, Bingcheng[1];Liang, Xinmao[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]Cent South Univ, Dept Pharm, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China

年份：2018

卷号：182

起止页码：500

外文期刊名：TALANTA

收录：;EI(收录号：20180704802328);WOS:【SCI-EXPANDED(收录号:WOS:000428229200064)】；

基金：The research was sponsored by National Natural Science Foundation of China (No. 21477037, 21322502) and the outstanding young talent cultivation fund of ECUST. BCY is grateful to Prof. Sandy Dasgupta in Texas University at Arlington for his fruitful guidance and helpful discussions for many years.

语种：英文

外文关键词：Hydrophilic interaction liquid chromatography; Stationary phase; Bleeding; Poly (vinyl alcohol); Cross-linker

摘要：A common drawback of silica-based polar stationary phase is obvious bleeding, probably resulting from silica dissolution in water attracted onto silica gel. Recently we have reported a polyvinyl alcohol (PVA) encapsulated silica gel stationary phase for hydrophilic interaction liquid chromatography (HILIC), which demonstrated good chromatographic performance. Although PVA coating outside could shield SiO2 inside from erosion by aqueous solution to a certain degree, obvious bleeding of such phase was still observed. To eliminate or reduce the bleeding level of the phase, fabricating multi-layers of PVA coating crosslinked with glutaraldehyde onto silica gel is performed (PVA-GA-Sil). similar to 20-fold decrease of the bleeding for PVA-GA-Sil can be achieved relative to bare silica gel, which is much lower than (or comparable to) commercial HILIC phases. PVA-GA-Sil also showed chromatographic performance and demonstrated superiority to commercial columns operated at gradient mode.