文献类型：期刊文献

英文题名：Probing the Intracellular Dynamics of Nitric Oxide and Hydrogen Sulfide Using an Activatable NIR II Fluorescence Reporter

作者：Zhu, Tianli[1];Ren, Ning[2];Liu, Xia[2];Dong, Yan[1];Wang, Rongchen[1];Gao, Jinzhu[1];Sun, Jie[1];Zhu, Ying[2];Wang, Lihua[2];Fan, Chunhai[3,4];Tian, He[1];Li, Jiang[2];Zhao, Chunchang[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Mat & Dynam Chem, Sch Chem & Mol Engn, Key Lab Adv Mat,Inst Fine Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol,Zhangjiang, Shanghai Adv Res Inst,Shanghai Inst Appl Phys,Div, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201210, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Frontiers Sci Ctr Transformat Mol, Shanghai 200240, Peoples R China;[4]Shanghai Jiao Tong Univ, Natl Ctr Translat Med, Shanghai 200240, Peoples R China

年份：2021

卷号：60

期号：15

起止页码：8450

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20211010027455);WOS:【SCI-EXPANDED(收录号:WOS:000624577900001)】；

基金：We gratefully acknowledge financial support by the National Natural Science Foundation of China (21672062, 21874043, 21788102, 22022410, 82050005, 21834007, 21934007 and 21775157), the Shanghai Municipal Science and Technology Major Project (Grant 2018SHZDZX03), the National Key Research and Development Program (2016YFA0201200), the Shanghai Municipal Science and Technology Commission (19JC1410300) and the Youth Innovation Promotion Association of CAS (Grant No. 2016236).

语种：英文

外文关键词：activatable probe; H2S; molecular probe; NIR II Fluorescence; NO

摘要：Understanding the complex interplay among gasotransmitters is of great significance but remains technically challenging. In this study, we present the design and synthesis of a dually responsive BOD-NH-SC reporter for probing the dynamic and alternating existence of NO and H2S in living cells. This designed reporter can repeatedly cycle S-nitrosation and transnitrosation reactions when successively treated with NO and H2S, thus affording the interchange of NIR fluorescence at 645 nm (NO) and NIR II fluorescence at 936 nm (H2S). In light of this unique fluorescence alternation between two colors, we synthesized water-soluble BOD-NH-SC dots to visualize the intracellular dynamics of NO and H2S. These molecular probes thus provide a toolbox to elucidate the interplaying roles of NO and H2S in the complex interaction networks of various signal transduction pathways.