文献类型：期刊文献

英文题名：Surface-Enhanced Raman Spectroscopy for Biomedical Applications: Recent Advances and Future Challenges

作者：Lin, Linley Li[1,2];Alvarez-Puebla, Ramon[3,4];Liz-Marzan, Luis M.[5,6,7,8];Trau, Matt[9,10];Wang, Jing[11];Fabris, Laura[12];Wang, Xiang[13];Liu, Guokun[14];Xu, Shuping[15];Han, Xiao Xia[15];Yang, Liangbao[16,17];Shen, Aiguo[18];Yang, Shikuan[19];Xu, Yikai[20];Li, Chunchun[21];Huang, Jinqing[22];Liu, Shao-Chuang[23];Huang, Jian-An[24,25,26];Srivastava, Indrajit[27,28];Li, Ming[29];Tian, Limei[30,31];Nguyen, Lam Bang Thanh[32];Bi, Xinyuan[1,2];Cialla-May, Dana[33,34,35];Matousek, Pavel[36,37];Stone, Nicholas[37];Carney, Randy P.[38];Ji, Wei[39];Song, Wei[15];Chen, Zhou[1,2];Phang, In Yee[40];Henriksen-Lacey, Malou[5,7];Chen, Haoran[1,2];Wu, Zongyu[1,2];Guo, Heng[30,31];Ma, Hao[13];Ustinov, Gennadii[33,34,35];Luo, Siheng[13];Mosca, Sara[36];Gardner, Benjamin[37];Long, Yi-Tao[23];Popp, Juergen[33,34,35];Ren, Bin[13];Nie, Shuming[41];Zhao, Bing[15];Ling, Xing Yi[32,40];Ye, Jian[1,2]

机构：[1]Shanghai Jiao Tong Univ, Peoples Hosp 6, Sch Med, Shanghai 200030, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200030, Peoples R China;[3]Univ Rovira & Virgili, Dept Quim Fis Inorgan, Tarragona 43007, Spain;[4]ICREA Inst Catalana Recerca & Estudis Avancats, Barcelona 08010, Spain;[5]Basque Res & Technol Alliance BRTA, CIC biomaGUNE, Donostia San Sebastian, Spain;[6]Univ Santiago de Compostela, Ikerbasque, Bilbao 48013, Spain;[7]Ctr Invest Cooperat Red Bioingn Biomat & Nanomed, Donostia San Sebastian, Spain;[8]Univ Vigo, CINBIO, Vigo, Spain;[9]Univ Queensland, Australian Inst Bioengn & Nanotechnol AIBN, Ctr Personalized Nanomed, Brisbane, Qld 4072, Australia;[10]Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia;[11]Fujian Normal Univ, Key Lab OptoElect Sci & Technol Med, Minist Educ, Fujian Prov Key Lab Photon Technol, Fuzhou 350117, Peoples R China;[12]Politecn Torino, Dept Appl Sci & Technol, I-10129 Turin, Italy;[13]Xiamen Univ, Coll Chem & Chem Engn,Innovat Lab Sci & Technol E, Collaborat Innovat Ctr Chem Energy Mat iChEM, Dept Chem,State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China;[14]Xiamen Univ, State Key Lab Marine Environm Sci, Fujian Prov Key Lab Coastal Ecol & Environm Studi, Ctr Marine Environm Chem & Toxicol,Coll Environm, Xiamen 361005, Peoples R China;[15]Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China;[16]Chinese Acad Sci, Hefei Inst Phys Sci, Inst Hlth & Med Technol, Hefei 230031, Anhui, Peoples R China;[17]Chinese Acad Sci, Hefei Canc Hosp, Dept Pharm, Hefei 230031, Anhui, Peoples R China;[18]Wuhan Text Univ, Sch Bioengn & Hlth, Wuhan 430200, Peoples R China;[19]Zhejiang Univ, Sch Mat Sci & Engn, Hangzhou 310027, Peoples R China;[20]East China Univ Sci & Technol, Feringa Nobel Prize Sci Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[21]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[22]Hong Kong Univ Sci & Technol, Dept Chem, Hong Kong 999077, Peoples R China;[23]Nanjing Univ, Mol Sensing & Imaging Ctr, Sch Chem & Chem Engn, Nanjing 210023, Peoples R China;[24]Univ Oulu, Fac Med, Res Unit Hlth Sci & Technol, Oulu 90220, Finland;[25]Univ Oulu, Fac Biochem & Mol Med, Dis Networks Res Unit, Oulu 90220, Finland;[26]Univ Oulu, Bioctr Oulu, Oulu 90220, Finland;[27]Texas Tech Univ, Dept Mech Engn, Lubbock, TX 79409 USA;[28]Texas Ctr Comparat Canc Res TC3R, Amarillo, TX 79106 USA;[29]Cent South Univ, Sch Mat Sci & Engn, Changsha 410083, Hunan, Peoples R China;[30]Texas A&M Univ, Dept Biomed Engn, College Stn, TX 77843 USA;[31]Texas A&M Univ, Ctr Remote Hlth Technol & Syst, College Stn, TX 77843 USA;[32]Nanyang Technol Univ, Sch Chem Chem Engn & Biotechnol, Singapore 637371, Singapore;[33]Leibniz Ctr Photon Infect Res LPI, Leibniz Inst Photon Technol, D-07745 Jena, Germany;[34]Friedrich Schiller Univ Jena, Inst Phys Chem IPC, Leibniz Ctr Photon Infect Res LPI, D-07743 Jena, Germany;[35]Friedrich Schiller Univ Jena, Abbe Ctr Photon ACP, Leibniz Ctr Photon Infect Res LPI, F-07743 Ger, France;[36]STFC Rutherford Appleton Lab, UKRI, Cent Laser Facil, Res Complex Harwell, Harwell Campus, Oxford OX11 0QX, Oxon, England;[37]Univ Exeter, Dept Phys & Astron, Exeter EX4 4QL, England;[38]Univ Calif Davis, Dept Biomed Engn, Davis, CA 95616 USA;[39]Northeast Forestry Univ, Coll Chem Chem Engn & Resource Utilizat, Harbin 145040, Peoples R China;[40]Jiangnan Univ, Sch Chem & Mat Engn, Key Lab Synthet & Biol Colloids, Int Joint Res Lab Nano Energy Composites, Wuxi 214122, Peoples R China;[41]Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA

年份：2025

卷号：17

期号：11

起止页码：16287

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20250917977475);WOS:【SCI-EXPANDED(收录号:WOS:001433015400001)】；

基金：We thank Qing He, Yaxuan Lu, and Han Shen for their help in preparing and organizing the manuscript. The authors are thankful for the following grants. Jian Ye, Linley Li Lin, and Xinyuan Bi: National Key Research and Development Program of China (No. 2024YFF1502600), National Natural Science Foundation of China (Nos. 82272054, 82372016, 623B2070), Science and Technology Commission of Shanghai Municipality (No. 24DIPA00300), Shanghai Jiao Tong University (Nos. YG2024LC09, YG2024QNA15). Aiguo Shen: National Natural Science Foundation of China (Nos. 22074109, 2247040793). Ramon Alvarez-Puebla: Projects PID2020-120306RB-I00 and PID2020-113704RB-I00 (funded by MCIN/AEI/10.13039/501100011033), PDC2021-121787-I00 (funded by MCIN/AEI/10.13039/501100011033 and European Union Next Generation EU/PRTR), 2017SGR883 (funded by Generalitat de Cataluna), and 2021PFR-URV-B2-02 (funded by Universitat Rovira i Virgili). Xing Yi Ling: Singapore National Research Foundation Investigatorship (NRF-NRFI08-2022-0011), Competitive Research Programme (NRF-CRP26-2021-0002). Limei Tian: Funding from the National Science Foundation (No. 1648451) and the National Institutes of Health (No. R35 GM147568). Luis M. Liz-Marzan: European Research Council (ERC Advanced Grant 787510, 4DbioSERS). Chunchun Li: Shanghai Pujiang Program (No. 23PJ1409000). Shuping Xu: National Natural Science Foundation of China (No. 22373041), Science and Technology Development Program Projects of Jilin Province (No. 20220101046JC). Jian-An Huang: Academy Research Fellow project, TwoPoreProSeq (No. 347652), and DigiHealth project (No. 326291), a strategic profiling project at the University of Oulu that is supported by the Academy of Finland and the University of Oulu. Xiao Xia Han and Bing Zhao: National Natural Science Foundation of China (Nos. 22073014, 22074051). Yikai Xu: Fundamental Research Funds for the Central Universities (No. JKJ01231812). Jinqing Huang: Research Grant Council of Hong Kong (No. 16309721). Yitao Long: National Natural Science Foundation of China (No. 2220407). Matt Trau: ARC Australian Laureate Fellowship (No. FL220100059). Jing Wang: National Natural Science Foundation of China (No. 12074069). Liangbao Yang: National Natural Science Foundation of China (No. 22474143). Guokun Liu: National Natural Science Foundation of China (No. 22272139). Pavel Matousek: "RaNT: Raman Nanotheranostics" EPSRC Programme Grant (EP/R020965/1) and EP/P012442/1. Randy P. Carney: National Institutes of Health (NIH) R01CA241666. Jurgen Popp: The Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) (No. 465289819).

语种：英文

外文关键词：Raman scattering; plasmonics; nanomedicine; metabolic detection; transmission Raman spectroscopy; SERSome

摘要：The year 2024 marks the 50th anniversary of the discovery of surface-enhanced Raman spectroscopy (SERS). Over recent years, SERS has experienced rapid development and became a critical tool in biomedicine with its unparalleled sensitivity and molecular specificity. This review summarizes the advancements and challenges in SERS substrates, nanotags, instrumentation, and spectral analysis for biomedical applications. We highlight the key developments in colloidal and solid SERS substrates, with an emphasis on surface chemistry, hotspot design, and 3D hydrogel plasmonic architectures. Additionally, we introduce recent innovations in SERS nanotags, including those with interior gaps, orthogonal Raman reporters, and near-infrared-II-responsive properties, along with biomimetic coatings. Emerging technologies such as optical tweezers, plasmonic nanopores, and wearable sensors have expanded SERS capabilities for single-cell and single-molecule analysis. Advances in spectral analysis, including signal digitalization, denoising, and deep learning algorithms, have improved the quantification of complex biological data. Finally, this review discusses SERS biomedical applications in nucleic acid detection, protein characterization, metabolite analysis, single-cell monitoring, and in vivo deep Raman spectroscopy, emphasizing its potential for liquid biopsy, metabolic phenotyping, and extracellular vesicle diagnostics. The review concludes with a perspective on clinical translation of SERS, addressing commercialization potentials and the challenges in deep tissue in vivo sensing and imaging.