文献类型：期刊文献

英文题名：Hydrophilic interaction liquid chromatography promotes the development of bio-separation and bio-analytical chemistry

作者：Sheng, Qianying[1];Liu, Meiyan[2];Lan, Minbo[1];Qing, Guangyan[3]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China

年份：2023

卷号：165

外文期刊名：TRAC-TRENDS IN ANALYTICAL CHEMISTRY

收录：;EI(收录号：20232514282102);WOS:【SCI-EXPANDED(收录号:WOS:001031599500001)】；

基金：& nbsp;This work was supported by the National Natural Science Foundation of China (21922411, and 22174138) , DICP Innovation Funding (DICP-I2020 08, I202243, and I202229) , the Dalian Outstanding Young Scientific Talent (2020RJ01) , and the National Key R & amp;D Program of China (Grant No. 2022YFC3400800) .

语种：英文

外文关键词：Hydrophilic interaction; HILIC materials; Bio-separation; Analysis; Glycoproteomics; Chromatography

摘要：Hydrophilic interaction liquid chromatography (HILIC) has inherent advantages in the separation of hydrophilic or strongly polar substances (e.g., saccharides, glycopeptides, metabolites, etc.) due to strong hydrophilic interactions, which represents an attractive complementary approach to the mainstreamed reversed phase LC. Significant improvement in sensitivity, analyte coverage, throughput, analysis speed, and resolution of HILIC largely promote the development of bio-separation and bio-analytical chemistry. Based on the theoretical guidance, a lot of functional hydrophilic materials have emerged, what follows is that people are more concerned about their innovations and breakthroughs. Meanwhile, the combina-tion of HILIC with various separation and characterization techniques (such as HILIC MS, multidimen-sional LC) present high separation efficiency towards complex samples in the fields of proteomics, metabolomics, food, and medicine industry. In this review the latest progress in HILIC in the past five years are summarized in order to provide a reliable and perfect guidance for future research in bio-separation. & COPY; 2023 Elsevier B.V. All rights reserved.