文献类型：期刊文献

英文题名：Engineering bioscaffolds for enzyme assembly

作者：Dong, Hao[1];Zhang, Wenxue[1];Zhou, Shengmin[1];Huang, Jiaofang[1];Wang, Ping[2]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA

年份：2021

卷号：53

外文期刊名：BIOTECHNOLOGY ADVANCES

收录：;EI(收录号：20210910009385);WOS:【SCI-EXPANDED(收录号:WOS:000723655800009)】；

基金：This work was sponsored by the National Natural Science Foundation of China (21636003, 21672065, 22077032, and 31872728), the International S&T Innovation Cooperation Key Project (2017YFE0129600), the National Science and Technology Major Project of the Ministry of Science and Technology of China (2020YFA0908900), the Natural Science Foundation of Shanghai (19ZR1477100), the Fundamental Research Funds for the Central Universities (22221818014), and the 111 Project (B18022).

语种：英文

外文关键词：Non-living bioscaffold; Living bioscaffold; Enzyme assembly; Biocompatibility; Regenerability

摘要：With the demand for green, safe, and continuous biocatalysis, bioscaffolds, compared with synthetic scaffolds, have become a desirable candidate for constructing enzyme assemblages because of their biocompatibility and regenerability. Biocompatibility makes bioscaffolds more suitable for safe and green production, especially in food processing, production of bioactive agents, and diagnosis. The regenerability can enable the engineered biocatalysts regenerate through simple self-proliferation without complex re-modification, which is attractive for continuous biocatalytic processes. In view of the unique biocompatibility and regenerability of bioscaffolds, they can be classified into non-living (polysaccharide, nucleic acid, and protein) and living (virus, bacteria, fungi, spore, and biofilm) bioscaffolds, which can fully satisfy these two unique properties, respectively. Enzymes assembled onto non-living bioscaffolds are based on single or complex components, while enzymes assembled onto living bioscaffolds are based on living bodies. In terms of their unique biocompatibility and regenerability, this review mainly covers the current advances in the research and application of non-living and living bioscaffolds with focus on engineering strategies for enzyme assembly. Finally, the future development of bioscaffolds for enzyme assembly is also discussed. Hopefully, this review will attract the interest of researchers in various fields and empower the development of biocatalysis, biomedicine, environmental remediation, therapy, and diagnosis.