文献类型：期刊文献

英文题名：Bifunctional alcohol/aldehyde dehydrogenase AdhE controls phospho-transferase system sugar utilization and virulence gene expression by interacting PtsH in Edwardsiella piscicida

作者：Mao, Qiaoqiao[1];Jiang, Jihao[1];Wu, Xiao[1];Ma, Yue[1];Zhang, Yuanxing[2,3];Zhao, Yanni[5];Zhang, Yibei[1,3];Wang, Qiyao[1,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China;[5]Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China

年份：2022

卷号：260

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20221511953274);WOS:【SCI-EXPANDED(收录号:WOS:000805437300002)】；

基金：This work was supported by grants from the National Key Research and Development Program of China, China (2018YFD0900504) , National Natural Science Foundation of China, China (32130108) , China Agriculture Research System of Ministry of Finance (MOF) and Ministry of Agriculture and Rural Affairs (MARA) , China (CARS-47) and Post-doctoral Science Foundation of China, China (2021M701206) . ZYN was supported by grants of National Natural Science Foundation of China, China (31800328) and Key Research Project of Shaanxi Provincial Science and Technology Department, China (2021NY-164) .

语种：英文

外文关键词：Alcohol; aldehyde dehydrogenase (AdhE); Edwardsiella piscicida; Sugar metabolism; Phospho-transferase system; Type III secretion system (T3SS)

摘要：The bifunctional alcohol/aldehyde dehydrogenase (AdhE), one of the key enzymes in the bacterial ethanol anaerobic fermentation pathway, is critical for appropriate expression of the genes for the utilization of carbon sources. Knowledge about its global roles in modulating gene expression and metabolomics remains limited. Edwardsiella bacteria includes several important zoonotic pathogenic species including Edwardsiella piscicida, a leading fish pathogen that causes severe economic losses in the aquaculture industry. It is well known to utilize few sugars. In this study, we showed that AdhE is involved in various processes including sugar utilization, bacteria growth, intracellular pH homeostasis, type III/VI secretion system (T3/T6SS) production, and survival in fish. Moreover, our unbiased metabolomics approaches revealed that AdhE modulates a large quantity of metabolic pathways, including amino acids, tricarboxylic acid (TCA) intermediates, sugar and fatty acids. Pull down and Co-immunoprecipitation (IP) analysis revealed that AdhE interacts with the phospho-transferase system component PtsH that supports the transform of its PTS sugars including mannose to mannose-6P, the established metabolic ligand modulating EvrA activity to control T3/T6SS expression. Collectively, AdhE appears to play important roles in bacterial adapting to the internal environment changes by regulating sugar metabolic pathways and bacterial virulence expression. These observations support a model in which AdhE acts a macromolecule hub accommodating proteins to modulate the PTS and other signaling cascades related to pathogenesis and environmental adaptation in bacterial pathogens, which may provide new perspectives for attempts to attenuate bacterial virulence.