文献类型：期刊文献

中文题名：氮源对庆大霉素合成与分泌的影响

英文题名：Influence of Nitrogen Sources on Synthesis and Secretion of Gentamicin

作者：张达力[1];储炬[1];李友荣[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2002

卷号：28

期号：4

起止页码：360

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2002447167714);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目 ( 39870 0 18

语种：中文

中文关键词：氮源;庆大霉素;合成;分泌;广谱抗生素;初始培养基;硫酸铵;硝酸钠;氨基酸

外文关键词：nitrogen source;gentamicin;synthesis;secretion

摘要：研究了无机与有机氮源对庆大霉素合成与分泌的影响。分别在初始培养基中及进入生物合成期的发酵液中添加硫酸铵、硝酸钠和各种氨基酸 ,初始培养基中添加硫酸铵浓度为 0 .1 g/ L时 ,总效价提高了 2 3 % ,同时 ,硫酸铵对庆大霉素组分也有一定影响。在培养至 2 4h添加 8g/ L硝酸钠 ,总效价提高 5 5 % ,而在初始培养基中分泌率提高了 3 3 %。在生物合成期添加 0 .8g/ L赖氨酸 ,总效价提高了 64%。
The effects of inorganic and organic nitrogen sources on synthesis and secretion of gentamicin in shake flask fermetation were studied. (NH 4) 2SO 4,NaNO 3 and various amino acids were added initially and in biosynthesis phase. The results showed that total potency increased by 23% when the concentration of (NH 4) 2SO 4 was 0.1g/L; component of gentamicin was also influenced by (NH 4) 2SO 4; total potency incresased by 55% when 8g/L NaNO 3 was added at 24h; secretion ratio increased by 33% when 8g/L NaNO 3 was added intially; total potency increased by 64% when 0.8g/L lysine was added in the biosynthesis phase. At the same time, verified experiment was done in the resting cell system.