文献类型：期刊文献

英文题名：A culture-dependent analysis of anaerobic methylotrophs in oil reservoir systems

作者：Huang, Lin-Yuan[1,2,3];Liu, Yi-Fan[1,2,3];Qin, Wan-Qi[1,2,3];Wang, Shi-Yi[1,2];Wang, Yuan-Hao[1,2];Zhou, Lei[1,2,3];Yang, Shi-Zhong[1,2,3];Gu, Ji-Dong[4,5];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr MEOR, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Guangdong Technion Israel Inst Technol, Environm Sci & Engn Grp, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China;[5]Guangdong Technion Israel Inst Technol, Guangdong Prov Key Lab Mat & Technol Energy Conver, Shantou 515063, Guangdong, Peoples R China

年份：2024

卷号：194

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20243016752062);WOS:【SCI-EXPANDED(收录号:WOS:001279822400001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 42173076, 42061134011 and 52074129) , the Natural Science Foundation of Shanghai (Grant No. 21ZR1417400) , Fundamental Research Funds for the Central Universities (No. JKJ01241714) and the Research Program of State Key Laboratory of Bioreactor Engineering to Y.F.L. and B.Z.M, the NSFC/RGC Joint Research Fund (No. 41161160560) to J.D.G.

语种：英文

外文关键词：Methyl compounds; Methylotrophic microorganisms; Methylotrophic methanogenesis; Oil reservoir; Methanol

摘要：Obtaining pure cultures and enrichment systems of methylotrophic anaerobic microorganisms capable of utilizing methyl compounds is critical to studying the carbon cycle in subsurface anoxic oil reservoirs. Cultureindependent methods have been instrumental in uncovering the rich diversity of microorganisms in oil reservoirs. However, there remains a notable scarcity of methylotrophic microorganisms from oil reservoirs obtained by culture-dependent methods. In this study, we used five different methyl compounds, namely methanol, methylamine, dimethylamine trimethylamine, and methyl sulfide, as the sole substrates to isolate methylutilizing microorganisms from oil reservoirs, and H2 was also added together with each of these methyl compounds in a separate isolation experiment, to facilitate H2-dependent methylotrophic growth and metabolism. Notably, the highest colony numbers in roll tubes were achieved when using methanol as the substrate. A total of 306 pure strains, representing eight genera were obtained, and these microorganisms have rarely been reported for their ecological roles in oil reservoir systems. Following isolation, each strain was tested for utilization of methanol as the sole carbon and energy sources after a second transfer, including headspace gas assay and microbial cell observations. In addition, enrichment cultures amended with each of the five different methyl compounds with or without the addition of H2 gas were established from four oil reservoir samples. Further experiments showed that the archaea enriched with methyl substrates from different oil reservoirs were almost all Methanobacteria, but after adding H2, the H2-dependent methylotrophic methanogen Ca. Methanomethylica was enriched in most of the enrichment cultures. On the contrary, the addition of H2 has less impact on the bacterial communities. The isolation of pure cultures has significantly enhanced our understanding of the diversity and ecophysiology of methylotrophic microorganisms in oil reservoirs. This study has provided useful insights into methyl-based methane generation within oil reservoirs, contributing to further understanding of the microbial ecology and carbon cycle in anoxic oil reservoirs.