文献类型：期刊文献

中文题名：水飞蓟全草不同部位的组成特征

英文题名：Structural features in composition of different parts of Silybum marianum(L.)Gaertn

作者：王芸[1];AZEEM Intisar[1];BOIMA KIAZOLU J[1];刘海燕[1];张维冰[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2010

卷号：28

期号：12

起止页码：1192

中文期刊名：色谱

外文期刊名：Chinese Journal of Chromatography

收录：CSTPCD;;Scopus;WOS:【ESCI(收录号:WOS:000435243400017)】；北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家自然科学基金项目(No.20675083);国家重点基础研究发展规划(“973”计划)项目(No.01CB5m202)

语种：中文

中文关键词：高效液相色谱;组分;水飞蓟;中草药

外文关键词：high performance liquid chromatography(HPLC);composition;Silybum marianum(L.)Gaertn;Chinese herbal medicine

摘要：为了考察水飞蓟全草不同部位的组成特征,采用不同的提取溶剂对水飞蓟的幼草、根、茎、叶、花、种子等不同部位提取后,通过高效液相色谱法(HPLC)进行分析。以Hypersil ODS2色谱柱(250mm×4.6mm,5μm)为分离柱,以50mmoL/L磷酸二氢钾溶液和甲醇为流动相,梯度洗脱,检测波长为254nm。在此条件下,水飞蓟不同部位的样品得到了很好的分离,发现不同部位组成差别很大,得到的组分数从幼草、种子、花、叶、根、茎依次减少。水飞蓟不同部位所含的组分极性有很大差别,不同提取方法得到的样品组成也有较大差别。实验结果可为水飞蓟全草的指纹图谱构建及应用开发提供实验依据。
To investigate the characters of different parts of Silybum marianum(L.)Gaertn,a high performance liquid chromatographic(HPLC)method was used to analyze the extracts of different parts(including tender sprouts,roots,stems,leaves,flowers,seeds,etc.)of Silybum marianum(L.)Gaertn with different solvents.There was much difference in the composition of different parts of Silybum marianum(L.)Gaertn.The sequence of the total number of components existed is as follows:tender sprouts,seeds,flowers,leaves,roots,stems.The obvious differences of polar components were observed not only in the nature of the compounds extracted by different solvents.The experiments will provide results for the development of fingerprint and application of Silybum marianum(L.)Gaertn.