文献类型：期刊文献

英文题名：Converting lignin derived phenolic aldehydes into microbial lipid by Trichosporon cutaneum

作者：Hu, Mingshan[1];Wang, Juan[1];Gao, Qiuqiang[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：281

起止页码：81

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20182605360537);WOS:【SCI-EXPANDED(收录号:WOS:000440561500010)】；

语种：英文

外文关键词：Trichosporon cutaneum; Lignin; Phenolic aldehydes; Microbial lipid; 4-hydroxybenzaldehyde

摘要：Lignin is one of the major components of lignocellulose biomass and chemically degrades into phenolic aldehydes including 4-hydroxybenzaldehyde, vanillin, and syringaldehyde. No lipid accumulation from the phenolic aldehydes by oleaginous microbes had been succeeded. Compared with vanillin and syringaldehyde, T. cutaneum ACCC 20271 have better tolerance to 4-hydroxybenzaldehyde. 4-Hydroxybenzaldehyde was found to be able as the substrate for lipid accumulation, while vanillin and syringaldehyde were only converted to less toxic phenolic alcohols and acids without observable lipid accumulation, perhaps due to the space shelling of methoxyl group(s) in the structures. A long term fed batch fermentation of 4-hydroxybenzaldehyde accumulated 0.85 g L-1 of lipid, equivalent to 0.039 g lipid per gram of 4-hydroxybenzaldehyde substrate, approximately 3.7 folds greater than the control without 4-hydroxybenzaldehyde addition. The fatty acid composition well met the need for biodiesel synthesis. The preliminary pathway from 4-hydroxybenzaldehyde to lipid was predicted. This study took the first experimental trial on utilizing phenolic aldehydes as the sole carbon sources for microbial lipid accumulation by T. cutaneum ACCC 20271.