文献类型：期刊文献

英文题名：Rhodamine 6G conjugated-quantum dots used for highly sensitive and selective ratiometric fluorescence sensor of glutathione

作者：Gui, Rijun[1];An, Xueqin[1];Su, Hongjuan[1];Shen, Weiguo[1];Zhu, Linyong[2];Ma, Xingyuan[3];Chen, Zhiyun[1];Wang, Xiaoyong[1]

机构：[1]E China Univ Sci & Technol, Coll Chem & Mol Engn, Dept Phys Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Fine Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：94

起止页码：295

外文期刊名：TALANTA

收录：;EI(收录号：20122115056194);WOS:【SCI-EXPANDED(收录号:WOS:000305167300045)】；

基金：This work was financially supported by the National Natural Science Foundation of China (nos. 20673059 and 20573056), the National High-Tech Research and Development Plan of China ("863" plan no. 2011AA06A107), the Nature Science Keystone Foundation of Shanghai (08jc1408100), the Fundamental Research Funds for the Central Universities of China (WK0913002) and the Nanotechnology Special Program Foundation of Shanghai (0652nm010).

语种：英文

外文关键词：Quantum dots; Fluorescence; Glutathione; Ratiometric sensor

摘要：Rhodamine 6G (R6G) and 3-mercaptopropionic acid (MPA) capped-CdTe quantum dots (QDs) were conjugated by electrostatic interactions in aqueous solution. The R6G-QDs conjugate was utilized as a photoluminescence (PL) ratiometric sensor for the detection of glutathione (GSH). In this method, intentional introduction of GSH destroyed the conjugation of R6G and QDs, and induced regular PL change of R6G-QDs conjugates due to the competitive chelation between GSH and MPA ligand on the surface of QDs. The ratio of PL intensity of R6G (I-R6G) to that of QDs (I-QDs) in this conjugate was near linear toward the concentration of GSH in the range from 0.05 to 80 mu M, and corresponding regression equation showed a good linear coefficient of 0.9954. The limit of detection of 15 nM in this proposed method was about 40-fold lower than that of other QDs-based PL sensors. Interferential experiments testified that R6G-QDs conjugates-based ratiometric PL sensor of GSH showed high selectivity over other related thiols and amino acids. Real sample assays further verified perfect analysis performance of the PL sensor of GSH. In comparison with conventional analytical techniques for the measurement of GSH, this ratiometric PL sensor was facile, economic, highly sensitive and selective. (C) 2012 Elsevier B.V. All rights reserved.