文献类型：期刊文献

英文题名：Oral ER-targeting near-infrared nanoprobes enable real-time intraoperative thoracic duct mapping in swine

作者：Zhang, Yutao[1];Li, Wennan[1];Yu, Qi[2];Subinuer, Maimaiti[2];Shen, Yaxing[2];Feng, Mingxiang[2];Guo, Zhiqian[1];Zhu, Wei-Hong[1]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem,Sch Chem & Mol Engn,Key Lab Adv Mat, Frontiers Sci Ctr Materiobiol & Dynam Chem,Joint I, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Shanghai 200237, Peoples R China;[2]Fudan Univ, Zhongshan Hosp, Dept Thorac Surg, Shanghai 200032, Peoples R China

年份：2026

卷号：69

期号：2

起止页码：1008

外文期刊名：SCIENCE CHINA-CHEMISTRY

收录：;EI(收录号：20254019260141);WOS:【SCI-EXPANDED(收录号:WOS:001582287200001)】；

基金：This work was supported by the National Natural Science Foundation of China (22225805, 81400681, 32394001, 32121005), the Shanghai Science and Technology Innovation Action Plan (22Y11907200, 23J21901600), and the Innovation Program of Shanghai Municipal Education Commission, Shanghai Municipal Health Commission (2024ZZ2025).

语种：英文

外文关键词：near-infrared dye; fluorescent probe; oral administration; cyanine; surgical navigation; thoracic duct; formulation

摘要：The thoracic duct (TD), the largest lymphatic vessel in the human body, plays a critical role in returning lymph to the circulatory system. However, its dynamic, distensible nature and concealed anatomical location make intraoperative visualization critically challenging and increase the risk of injury. Real-time, high-resolution assessment of TD leaks remains an urgent clinical need. Here, we present a breakthrough molecular engineering strategy that leverages an intestinally lipophilic fluorescent formulation for dynamic in vivo TD imaging. Our rationally designed cyanine derivative IR790+, known for its rapid membrane permeability and endoplasmic reticulum (ER) targeting localization, demonstrates unprecedented chylomicron affinity, which subsequently transports the dye through the lymphatic system to the TD. Notably, dynamic, high-contrast intraoperative TD imaging is achieved from rat models to swine models. Administered orally as near-infrared (NIR) fluorescent contrast agent, this ultra-stable IR790+@oil formulation, engineered via flash nanoprecipitation, surpasses conventional counterparts by enabling non-invasive, real-time identification of TD. Intriguingly, this first-reported ER-targeting NIR formulation, delivered orally, represents a paradigm shift in fluorescence-guided surgery, significantly improving intraoperative accuracy.