文献类型：期刊文献

英文题名：Pattern recognition receptors involved in the inflammatory attenuating effects of soybean isoflavone in β-amyloid peptides 1-42 treated rats

作者：Yuan, Linhong[1,3];Zhou, Xin[1];Li, Dan[2];Ma, Weiwei[1];Yu, Huanling[1];Xi, Yuandi[1];Xiao, Rong[1,3]

机构：[1]Capital Med Univ, Dept Nutr & Food Hyg, Beijing, Peoples R China;[2]E China Univ Sci & Technol, Shanghai 200237, Peoples R China;[3]Beijing Municipal Key Lab Clin Epidemiol, Beijing, Peoples R China

年份：2012

卷号：506

期号：2

起止页码：266

外文期刊名：NEUROSCIENCE LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000300200400019)】；

基金：This work was supported by the grants from National Natural Science Foundation of China (No. 30972470), the National High Technology Research and Development Program of China (No. 2010AA023003), Beijing Municipal Natural Science Foundation (No. 7102015) and Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the jurisdiction of Beijing Municipality (PHR201006112), Beijing Municipal Foundation for the Excellent Talents (No. 2011D005018000011).

语种：英文

外文关键词：Pattern recognition receptors; Soybean isoflavone; Neuroinflammation; beta-Amyloid peptides1-42

摘要：Pattern recognition receptors (PRRs) play important roles in the inflammatory responses to Alzheimer's disease (AD). Our previous study indicated that soybean isoflavone (SIF) exhibited anti-inflammatory effect in rats treated by beta-amyloid peptides1-42 (A beta 1-42). In present study, we further detected the effects of SIF against inflammation caused by A beta 1-42 treatment in rats. Serum inflammatory mediators and neurotrophic factors including transforming growth factor-beta (TGF-beta), inducible nitric oxide synthase (iNOS), brain-derived neurotrophic factor (BDNF) and S100 beta were detected by enzyme-like immunosorbent assay (ELISA). Reverse transcription-polymerase chain reaction (RT-PCR) and western blot methods were applied for detecting mRNA and protein expression of interleukin-1 beta (IL-1 beta), iNOS, tumor necrosis factor-a (TNF-alpha), TGF-beta, BDNF, S100 beta, myeloid differentiation factor88 (Myd88), Toll-like receptor2 (TLR2), formyl peptide receptors (FPRs), inhibitor KB kinase (IKK) and inhibitor KB-alpha (IKB-alpha) in rat's brain tissue. Our results indicated that SIF could reduce the production of IL-1 beta, TNF-a and iNOS induced by A beta 1-42 in serum and brain of rats. SIF also significantly reversed A beta 1-42-induced up-regulation of TLR2, FPR, Myd88, IKK and decreased IKB-a mRNA and protein expressions in rats. These results suggested that TLR2 and FPR might involve in the inflammatory process induced by A beta 1-42 treatment, and SIF was an efficiency compound in reversing the inflammation caused by A beta 1-42 treatment. (C) 2011 Elsevier Ireland Ltd. All rights reserved.