文献类型：期刊文献

英文题名：The neuroprotective effect of picroside II from Hu-Huang-Lian against oxidative stress

作者：Li, Ting; Liu, Jian-Wen; Zhang, Xiao-Dong; Guo, Ming-Chuan; Ji, Guang

机构：[1]E China Univ Sci & Technol, Sch Pharm, Dept Pharmacol, State Key Lab Bioreactor Engn, Mailbox 268,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Univ TCM, Longhua Hosp, Lab Liver Dis, Shanghai 200032, Peoples R China

年份：2007

卷号：35

期号：4

起止页码：681

外文期刊名：AMERICAN JOURNAL OF CHINESE MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000249451000013)】；

语种：英文

外文关键词：neuroprotection; oxidative stress; pricroside II; Hu-Huang-Lian; aluminium; glutamate

摘要：Picroside II is an active constituent extracted from the traditional Chinese medicine (TCM) Hu-Huang-Lian. To evaluate the neuroprotective effect of picroside II, PC12 cells were treated with glutamate in vitro and male ICR mice were treated with AlCl. in vivo. Pretreatment of PC12 cells with picroside II could enhance the cell viability and decrease the level of intracellular reactive oxygen species (ROS) induced by glutamate. By DNA fragmentation and flow cytometry assay, picroside II (1.2 mg/ml) significantly prevented glutamate-induced cell apoptosis. In the animal study, amnesia was induced in mice by AlCl3 (100 mg/kg/d, i.v.). Pricroside II, at the dose of 20 and 40 mg/kg/d (i.g.), markedly ameliorated AlCl3-induced learning and memory dysfunctions and attenuated AlCl3-induced histological changes. This was associated with the significant increased superoxide dismutase (SOD) activity in the brain of experimental mice. All these results indicated that picroside II possessed the therapeutic potential in protecting against neurological injuries damaged by oxidative stress.