文献类型：期刊文献

英文题名：RNAi-Based Molecular Biopesticide: Inspired by Nature, Precision by Science, and Harmony to the Ecosystem

作者：Fan, Chunyu[1];Li, Zekai[1];Xu, Zhiping[1];Yang, Yangyang[1];Qian, Xuhong[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2026

卷号：74

期号：9

起止页码：7367

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20261120249190);WOS:【SCI-EXPANDED(收录号:WOS:001701916400001)】；

基金：This work is funded by the Shanghai Agricultural Science and Technology Innovation Project (T20252013 and K2025007) and the National Key Research and Development Program (2023YFD1700300 and 2022YFD1700400).

语种：英文

外文关键词：RNA interference; biopesticides; crop protection; nanopesticides

摘要：RNA interference (RNAi)-based molecular biopesticides represent a transformative approach to sustainable crop protection. By harnessing a natural gene silencing mechanism, these next-generation pesticides offer unparalleled species specificity, potentially overcoming the limitations of broad-spectrum chemicals. This review systematically outlines the research and development framework for RNA biopesticides. We explore the core processes of target gene discovery, RNA design with the use of computational tools, and scalable production methods. A significant focus is placed on delivery strategies, including nanocarriers and carrier-free systems. Furthermore, we critically discuss the biosafety considerations and ecological impacts of deploying RNAi agents in the environment. This review synthesizes how RNA biopesticides, inspired by nature and refined by scientific precision, can be integrated into agricultural ecosystems for harmonious and effective crop protection.